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  • © 2017

Lipidomics

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 125)

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6946-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 169.99
Price excludes VAT (USA)
Hardcover Book USD 169.99
Price excludes VAT (USA)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
  2. Introduction and Overview of Lipidomic Strategies

    • William J. Griffiths, Yuqin Wang
    Pages 1-11
  3. MALDI-Imaging Mass Spectrometry of Brain Lipids

    • Norelle C. Wildburger
    Pages 45-59
  4. Ion-Mobility Mass Spectrometry for Lipidomics Applications

    • Giuseppe Paglia, Bindesh Shrestha, Giuseppe Astarita
    Pages 61-79
  5. Quantification of Endogenous Endocannabinoids by LC-MS/MS

    • Mesut Bilgin, Andrej Shevchenko
    Pages 99-107
  6. Mass Spectrometric Analysis of Lipid Hydroperoxides

    • Tânia Melo, Elisabete Maciel, Ana Reis, Pedro Domingues, M. Rosário M. Domingues
    Pages 133-146
  7. CE Analysis of Phospholipid Headgroups

    • Václav Matěj Bierhanzl, Martina Riesová, Gabriela Seydlová, Radomír Čabala
    Pages 159-161
  8. HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids

    • Tatiana Kondakova, Nadine Merlet Machour, Cécile Duclairoir Poc
    Pages 163-173
  9. Global UHPLC/HRMS Lipidomics Workflow for the Analysis of Lymphocyte Suspension Cultures

    • Candice Z. Ulmer, Richard A. Yost, Timothy J. Garrett
    Pages 175-185
  10. Ambient Lipidomic Analysis of Brain Tissue Using Desorption Electrospray Ionization (DESI) Mass Spectrometry

    • Valentina Pirro, Alan K. Jarmusch, Christina R. Ferreira, R. Graham Cooks
    Pages 187-210
  11. Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids

    • Alexandra C. Kendall, Anna Nicolaou
    Pages 211-228
  12. Back Matter

    Pages 243-248

About this book

This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells (lymphocytes and oocytes).  Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility);  derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.

Keywords

  • Lipids
  • Mass Spectrometry Imaging
  • Oxysterols
  • Plasmenyl Glycerophospholipids
  • biomarkers

Editors and Affiliations

  • Metabolomics Unit, Lincoln Memorial University, Harrogate, USA

    Paul Wood

Bibliographic Information

  • Book Title: Lipidomics

  • Editors: Paul Wood

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-6946-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6944-9

  • Softcover ISBN: 978-1-4939-8347-6

  • eBook ISBN: 978-1-4939-6946-3

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 248

  • Number of Illustrations: 34 b/w illustrations, 61 illustrations in colour

  • Topics: Neuroscience

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6946-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 169.99
Price excludes VAT (USA)
Hardcover Book USD 169.99
Price excludes VAT (USA)