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  • © 2017

The Immune Synapse

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1584)

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6881-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xiii
  2. The Immune Synapse: Past, Present, and Future

    • Michael L. Dustin, Cosima T. Baldari
    Pages 1-5
  3. Analyzing Actin Dynamics at the Immunological Synapse

    • Katarzyna I. Jankowska, Janis K. Burkhardt
    Pages 7-29
  4. Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse

    • Noelia Blas-Rus, Eugenio Bustos-Morán, Francisco Sánchez-Madrid, Noa B. Martín-Cófreces
    Pages 31-49
  5. Analyzing the Dynamics of Signaling Microclusters

    • Akiko Hashimoto-Tane, Tadashi Yokosuka, Takashi Saito
    Pages 51-64
  6. Reconstitution of TCR Signaling Using Supported Lipid Bilayers

    • Xiaolei Su, Jonathon A. Ditlev, Michael K. Rosen, Ronald D. Vale
    Pages 65-76
  7. Studying the Dynamics of TCR Internalization at the Immune Synapse

    • Enrique Calleja, Balbino Alarcón, Clara L. Oeste
    Pages 89-99
  8. Imaging Vesicular Traffic at the Immune Synapse

    • Jérôme Bouchet, Iratxe del Río-Iñiguez, Andrés Alcover
    Pages 129-142
  9. Analysis of TCR/CD3 Recycling at the Immune Synapse

    • Laura Patrussi, Cosima T. Baldari
    Pages 143-155
  10. Mathematical Modeling of Synaptic Patterns

    • Anastasios Siokis, Philippe A. Robert, Michael Meyer-Hermann
    Pages 171-182
  11. Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy

    • Valarie A. Barr, Jason Yi, Lawrence E. Samelson
    Pages 183-206
  12. Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes

    • Lining Ju, Yunfeng Chen, Muaz Nik Rushdi, Wei Chen, Cheng Zhu
    Pages 231-258
  13. Spatial Control of Biological Ligands on Surfaces Applied to T Cell Activation

    • Haogang Cai, David Depoil, James Muller, Michael P. Sheetz, Michael L. Dustin, Shalom J. Wind
    Pages 307-331
  14. Probing Synaptic Biomechanics Using Micropillar Arrays

    • Weiyang Jin, Charles T. Black, Lance C. Kam, Morgan Huse
    Pages 333-346

About this book

This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists and, at a more general level, to cell biologists, biophysicists and computational biologists.

Keywords

  • T-cell
  • immune synapse
  • vesicular traffic
  • B cell
  • mast cell immune synapses
  • immune interactions

Editors and Affiliations

  • Department of Life sciences, University of Siena, Siena, Italy

    Cosima T. Baldari

  • University of Oxford, Kennedy Institute of Rheumatology, Headington, Oxford, United Kingdom

    Michael L. Dustin

About the editors



Bibliographic Information

  • Book Title: The Immune Synapse

  • Book Subtitle: Methods and Protocols

  • Editors: Cosima T. Baldari, Michael L. Dustin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6881-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • License: CC BY

  • Hardcover ISBN: 978-1-4939-6879-4Published: 05 March 2017

  • Softcover ISBN: 978-1-4939-8327-8Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6881-7Published: 01 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 588

  • Number of Illustrations: 25 b/w illustrations, 89 illustrations in colour

  • Topics: Immunology

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6881-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)