© 2017

Synthetic Antibodies

Methods and Protocols

  • Thomas Tiller


  • Includes cutting-edge methods and protocols for the study of synthetic antibodies

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1575)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Amino Acid-Based Synthetic Antibodies

    1. Front Matter
      Pages 1-1
    2. Xiaoying Zhang, Thirumalai Diraviyam
      Pages 3-13
    3. Mieko Kato, Yoshiro Hanyu
      Pages 31-44
    4. Michael W. Traxlmayr, Eric V. Shusta
      Pages 45-65
    5. Yvonne Stark, Sophie Venet, Annika Schmid
      Pages 67-91
    6. Marcin Paduch, Anthony A. Kossiakoff
      Pages 93-119
    7. Emilie Renaud, Pierre Martineau, Laurence Guglielmi
      Pages 165-174
    8. Helena Persson, Charlotta Preger, Edyta Marcon, Johan Lengqvist, Susanne Gräslund
      Pages 175-187
    9. Kai Zhang, Stephen J. Demarest, Xiufeng Wu, Jonathan R. Fitchett
      Pages 197-213
    10. Wouter P. R. Verdurmen, Marigona Mazlami, Andreas Plückthun
      Pages 223-236
    11. Maria Wendt, Guido Cappuccilli
      Pages 237-250
  3. Nucleotide-Based Synthetic Antibodies: Aptamers

    1. Front Matter
      Pages 251-251
    2. Nam Nguyen Quang, Anna Miodek, Agnes Cibiel, Frédéric Ducongé
      Pages 253-272

About this book


This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.


Amino acid based antibodies Affinity reagents Aptamers Nucleotides Moleculary imprinted polymers Immunoglobulin Scaffold engineering

Editors and affiliations

  • Thomas Tiller
    • 1
  1. 1.MorphoSys AG, Discovery Alliances & TechnologiesPlaneggGermany

Bibliographic information

  • Book Title Synthetic Antibodies
  • Book Subtitle Methods and Protocols
  • Editors Thomas Tiller
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-6855-8
  • Softcover ISBN 978-1-4939-8319-3
  • eBook ISBN 978-1-4939-6857-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 420
  • Number of Illustrations 28 b/w illustrations, 74 illustrations in colour
  • Topics Immunology
  • Buy this book on publisher's site