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RNA Methylation

Methods and Protocols

  • Book
  • © 2017


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material:

Part of the book series: Methods in Molecular Biology (MIMB, volume 1562)

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About this book

This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.


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Table of contents (20 protocols)

  1. Modifications in Abundant RNA Species

  2. Analysis of N6-Methyladenosine

  3. Analysis of 5-Methylcytosine

  4. Further RNA Modifications

Editors and Affiliations

  • Division of Molecular Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria

    Alexandra Lusser

Bibliographic Information

  • Book Title: RNA Methylation

  • Book Subtitle: Methods and Protocols

  • Editors: Alexandra Lusser

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6805-3Published: 28 March 2017

  • Softcover ISBN: 978-1-4939-8304-9Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6807-7Published: 27 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 304

  • Number of Illustrations: 28 b/w illustrations, 34 illustrations in colour

  • Topics: Human Genetics

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