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  • © 2017

Xylem

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1544)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6722-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.99
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Development

    1. Front Matter

      Pages 1-1
    2. Experimental and Theoretical Methods to Approach the Study of Vascular Patterning in the Plant Shoot

      • Norma Fàbregas, Pau Formosa-Jordan, Marta Ibañes, Ana I. Caño-Delgado
      Pages 3-19
    3. Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues

      • Sacha Escamez, Benjamin Bollhöner, Hannele Tuominen
      Pages 27-36
    4. Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis

      • Delphine Ménard, Henrik Serk, Raphaël Decou, Edouard Pesquet
      Pages 37-57
    5. Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

      • Masato Saito, Alif Meem Nurani, Yuki Kondo, Hiroo Fukuda
      Pages 59-65
  3. Imaging

    1. Front Matter

      Pages 75-75
    2. Live Imaging of Developing Xylem In Planta

      • Raymond Wightman
      Pages 77-82
    3. Immunolocalization in Secondary Xylem of Populus

      • Suzanne Gerttula, Andrew Groover
      Pages 83-90
    4. Vascular Morphodynamics During Secondary Growth

      • Pierre Barbier de Reuille, Laura Ragni
      Pages 103-125
    5. Using CellProfiler to Analyze and Quantify Vascular Morphology

      • Liam Campbell, Manoj Kumar, Simon Turner
      Pages 179-189
  4. Composition

    1. Front Matter

      Pages 191-191
    2. Lignin Analysis by HPLC and FTIR

      • Jorge Reyes-Rivera, Teresa Terrazas
      Pages 193-211
    3. Carbohydrate Composition Analysis in Xylem

      • Baocai Zhang, Yihua Zhou
      Pages 213-222

About this book

This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue.  Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.

Keywords

  • live imaging
  • computational analysis
  • xylem composition
  • imaging techniques
  • Xylem development
  • gene regulation
  • cellular systems

Editors and Affiliations

  • Department of Biosciences, Durham University, Durham, United Kingdom

    Miguel de Lucas, J. Peter Etchhells

Bibliographic Information

  • Book Title: Xylem

  • Book Subtitle: Methods and Protocols

  • Editors: Miguel de Lucas, J. Peter Etchhells

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6722-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • License: CC BY

  • Hardcover ISBN: 978-1-4939-6720-9

  • Softcover ISBN: 978-1-4939-8282-0

  • eBook ISBN: 978-1-4939-6722-3

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 262

  • Number of Illustrations: 13 b/w illustrations, 37 illustrations in colour

  • Topics: Plant Science

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6722-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.99
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)