Oral Biology

Molecular Techniques and Applications

  • Gregory J. Seymour
  • Mary P. Cullinan
  • Nicholas C.K. Heng
Part of the Methods in Molecular Biology book series (MIMB, volume 1537)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Saliva and Other Oral Fluids

    1. Front Matter
      Pages 1-1
    2. Blanca Majem, Feng Li, Jie Sun, David T. W. Wong
      Pages 17-36
    3. Iain L. C. Chapple, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Melissa M. Grant
      Pages 61-77
    4. Horst Joachim Schirra, Pauline J. Ford
      Pages 79-105
    5. Bruce J. Baum, Sandra Afione, John A. Chiorini, Ana P. Cotrim, Corinne M. Goldsmith, Changyu Zheng
      Pages 107-123
  3. Molecular Biosciences

    1. Front Matter
      Pages 125-125
    2. José F. Siqueira Jr., Isabela N. Rôças
      Pages 127-138
    3. Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, Ann R. Holmes
      Pages 165-190
    4. Mª José Marin, Elena Figuero, David Herrera, Mariano Sanz
      Pages 191-202
    5. Gabriela Salvadori, Roger Junges, Rabia Khan, Heidi A. Åmdal, Donald A. Morrison, Fernanda C. Petersen
      Pages 219-232
    6. Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Åmdal, Fernanda C. Petersen, Donald A. Morrison
      Pages 233-247
    7. Aniruddha Chatterjee, Euan J. Rodger, Ian M. Morison, Michael R. Eccles, Peter A. Stockwell
      Pages 249-277
    8. Aniruddha Chatterjee, Euan J. Rodger, Peter A. Stockwell, Gwenn Le Mée, Ian M. Morison
      Pages 279-298

About this book


This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.


saliva mass spectrometry NMR-based metabolomics gingival fluid oral microbial communities quantitative real-time PCR epigenetics DNA methylation oral diseases

Editors and affiliations

  • Gregory J. Seymour
    • 1
  • Mary P. Cullinan
    • 2
  • Nicholas C.K. Heng
    • 3
  1. 1.University of Otago Fac. DentistryDunedinNew Zealand
  2. 2.Department of Oral SciencesUniversity of Otago Faculty of DentistryDunedinNew Zealand
  3. 3.Sir John Walsh Research InstituteUniversity of Otago Faculty of DentistryDunedinNew Zealand

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6685-1
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6683-7
  • Online ISBN 978-1-4939-6685-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book