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Bacterial Pathogenesis

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Pontus Nordenfelt

    1. Department of Clinical Sciences, Lund University, Division of Infection Medicine, Lund, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Mattias Collin

    1. Department of Clinical Sciences, Lund University, Division of Infection Medicine, Lund, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1535)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
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  2. Identification and Characterization of Bacterial Effector Molecules

    1. Front Matter

      Pages 1-1
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    2. Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors

      • Rolf Lood, Inga-Maria Frick
      Pages 3-15

    3. Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics

      • Christofer Karlsson, Johan Teleman, Johan Malmström
      Pages 17-24

    4. Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein–Metabolite Interactions in Bacteria

      • Mona W. Orr, Vincent T. Lee
      Pages 25-41

    5. Identifying Bacterial Immune Evasion Proteins Using Phage Display

      • Cindy Fevre, Lisette Scheepmaker, Pieter-Jan Haas
      Pages 43-61

  4. Genetics and Phylogenetics of Bacterial Pathogens

    1. Front Matter

      Pages 95-95
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    2. Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities

      • Christian F. P. Scholz, Anders Jensen
      Pages 97-107

    3. Reconstructing the Ancestral Relationships Between Bacterial Pathogen Genomes

      • Caitlin Collins, Xavier Didelot
      Pages 109-137

  5. Bacterial Imaging Approaches and Related Techniques

    1. Front Matter

      Pages 139-139
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    2. Making Fluorescent Streptococci and Enterococci for Live Imaging

      • Sarah Shabayek, Barbara Spellerberg
      Pages 141-159

    3. Computer Vision-Based Image Analysis of Bacteria

      • Jonas Danielsen, Pontus Nordenfelt
      Pages 161-172

    4. Assessing Vacuolar Escape of Listeria Monocytogenes

      • Juan J. Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, Anne Danckaert et al.
      Pages 173-195

    5. Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy

      • Amy L. Bottomley, Lynne Turnbull, Cynthia B. Whitchurch, Elizabeth J. Harry
      Pages 197-209

    6. Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures

      • Matthias Mörgelin
      Pages 211-217

    7. Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes

      • Assaf Raz
      Pages 219-228

    8. Antibody Guided Molecular Imaging of Infective Endocarditis

      • Kenneth L. Pinkston, Peng Gao, Kavindra V. Singh, Ali Azhdarinia, Barbara E. Murray, Eva M. Sevick-Muraca et al.
      Pages 229-241
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Keywords

About this book

This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Editors and Affiliations

  • Department of Clinical Sciences, Lund University, Division of Infection Medicine, Lund, Sweden

    Pontus Nordenfelt, Mattias Collin

Bibliographic Information

  • Book Title: Bacterial Pathogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Pontus Nordenfelt, Mattias Collin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6673-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6671-4Published: 02 December 2016

  • Softcover ISBN: 978-1-4939-8268-4Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6673-8Published: 01 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 357

  • Number of Illustrations: 23 b/w illustrations, 48 illustrations in colour

  • Topics: Microbiology

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