Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1532)
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Table of contents (19 protocols)
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Front Matter
About this book
This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for theanalysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Keywords
- EBV-associated diseases
- EBV-epithelial cell interactions
- purification-mass spectrometry methods
- 3D telomere FISH
- high-throughput RNA sequencing
- qPCR and nuclear run-on assay
- chromatin immunoprecipitation coupled sequencing (ChIP-Seq)
- Zta-DNA interactions
- tissue culture
- post-transplant lymphoproliferative diseases (PTLD)
- Good Manufacturing Practice-complaint
- infectious diseases
Reviews
Editors and Affiliations
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University of Szeged, Szeged, Hungary
Janos Minarovits
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University of Regensburg, Regensburg, Germany
Hans Helmut Niller
Bibliographic Information
Book Title: Epstein Barr Virus
Book Subtitle: Methods and Protocols
Editors: Janos Minarovits, Hans Helmut Niller
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6655-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6653-0Published: 22 November 2016
Softcover ISBN: 978-1-4939-8262-2Published: 04 May 2018
eBook ISBN: 978-1-4939-6655-4Published: 21 November 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 269
Number of Illustrations: 1 b/w illustrations, 21 illustrations in colour
Topics: Infectious Diseases