Type 3 Secretion Systems

Methods and Protocols

  • Matthew L. Nilles
  • Danielle L. Jessen Condry

Part of the Methods in Molecular Biology book series (MIMB, volume 1531)

Table of contents

  1. Front Matter
    Pages i-x
  2. Danielle L. Jessen Condry, Matthew L. Nilles
    Pages 1-10
  3. Matthew S. Francis, Ayad A. A. Amer, Debra L. Milton, Tiago R. D. Costa
    Pages 11-31
  4. Matthew L. Nilles, Danielle L. Jessen Condry, Patrick Osei-Owusu
    Pages 61-70
  5. Philip R. Adam, Michael L. Barta, Nicholas E. Dickenson
    Pages 81-91
  6. Danielle L. Jessen Condry, Matthew L. Nilles
    Pages 93-99
  7. Rachel M. Olson, Deborah M. Anderson
    Pages 101-109
  8. Sabine Drehkopf, Jens Hausner, Michael Jordan, Felix Scheibner, Ulla Bonas, Daniela Büttner
    Pages 121-139
  9. Kyaw Aung, Xiufang Xin, Christy Mecey, Sheng Yang He
    Pages 141-153
  10. Peter L. Knopick, David S. Bradley
    Pages 165-172
  11. Michael L. Barta, Philip R. Adam, Nicholas E. Dickenson
    Pages 173-181
  12. Travis D. Alvine, Patrick Osei-Owusu, Danielle L. Jessen Condry, Matthew L. Nilles
    Pages 183-191
  13. Danielle L. Jessen Condry, Matthew L. Nilles
    Pages 203-211

About this book

Introduction

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.

Keywords

site-directed mutagenesis interactions of T3S components heterologous proteins effector translocation immune responses needle proteins

Editors and affiliations

  • Matthew L. Nilles
    • 1
  • Danielle L. Jessen Condry
    • 2
  1. 1.Department of Biomedical SciencesSchool of Medicine and Health Sciences University of North DakotaGrand ForksUSA
  2. 2.Department of Biomedical SciencesSchool of Medicine and Health Sciences University of North DakotaGrand ForksUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6649-3
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6647-9
  • Online ISBN 978-1-4939-6649-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book