© 2017


Methods and Protocols

  • Peter Sass


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1520)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Production and Design

    1. Front Matter
      Pages 1-1
    2. Martina Adamek, Marius Spohn, Evi Stegmann, Nadine Ziemert
      Pages 23-47
    3. Henrik Harms, Gabriele M. König, Till F. Schäberle
      Pages 49-61
    4. Georgios Daletos, Elena Ancheeva, Raha S. Orfali, Victor Wray, Peter Proksch
      Pages 63-83
    5. Wenbo Yu, Alexander D. MacKerell Jr.
      Pages 85-106
    6. Alexander Zipperer, Dorothee Kretschmer
      Pages 107-118
  3. Mode of Action

    1. Front Matter
      Pages 119-119
    2. Miriam Wilmes, Hans-Georg Sahl
      Pages 133-143
    3. Kathi Scheinpflug, Oxana Krylova, Henrik Strahl
      Pages 159-174
    4. Allan H. Pang, Sylvie Garneau-Tsodikova, Oleg V. Tsodikov
      Pages 175-200
    5. Yu Chen, Xin Liu, Nancy Wu, Carol A. Fierke
      Pages 201-225
    6. Christina E. Lünse, Günter Mayer
      Pages 227-235
    7. Mike Gajdiss, Michael Türck, Gabriele Bierbaum
      Pages 247-259
  4. Response and Susceptibility

    1. Front Matter
      Pages 261-261
    2. Shingo Suzuki, Takaaki Horinouchi, Chikara Furusawa
      Pages 263-279

About this book


This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies.  Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research. 


antibacterial compounds antibiotic biosynthesis clusters ligand based drug design compound-target interactions bacterial membrane

Editors and affiliations

  • Peter Sass
    • 1
  1. 1.Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive CompoundsUniversity of TübingenTübingenGermany

Bibliographic information

  • Book Title Antibiotics
  • Book Subtitle Methods and Protocols
  • Editors Peter Sass
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-6632-5
  • Softcover ISBN 978-1-4939-8255-4
  • eBook ISBN 978-1-4939-6634-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 358
  • Number of Illustrations 57 b/w illustrations, 21 illustrations in colour
  • Topics Microbiology
  • Buy this book on publisher's site