MicroRNA Profiling

Methods and Protocols

  • Sweta Rani

Part of the Methods in Molecular Biology book series (MIMB, volume 1509)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Anchal Vishnoi, Sweta Rani
    Pages 1-10
  3. John Nolan, Raymond L. Stallings, Olga Piskareva
    Pages 11-16
  4. K. K. Sabu, Fasiludeen Nadiya, Narayanannair Anjali
    Pages 25-36
  5. Melissa Daly, Lorraine O’Driscoll
    Pages 37-46
  6. Irene K. Oglesby, Paul J. McKiernan
    Pages 57-69
  7. Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall, Eva M. Jimenez-Mateos
    Pages 85-91
  8. Hanan Elshelmani, Sweta Rani
    Pages 93-113
  9. Rahul Bhome, Rebecca Goh, Karen Pickard, Massimiliano Mellone, A. Emre Sayan, Alex Mirnezami
    Pages 115-122
  10. Killian P. O’Brien, Eimear Ramphul, Linda Howard, William M. Gallagher, Carmel Malone, Michael J. Kerin et al.
    Pages 123-139
  11. Sadhbh O’Neill, Lorraine O’Driscoll
    Pages 141-149
  12. Sinéad T. Aherne, Nga T. Lao
    Pages 151-160
  13. Carmela Dell’Aversana, Cristina Giorgio, Lucia Altucci
    Pages 169-183
  14. Duncan Kilburn, Yunke Song, Tza-Huei Wang, Kelvin J. Liu
    Pages 185-193
  15. James A. L. Brown, Emer Bourke
    Pages 195-208
  16. Luis E. León, Sebastián D. Calligaris
    Pages 209-220

About this book

Introduction

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Keywords

human diseases cell viability and proliferation RNA isolation formalin fixed paraffin embedded (FFPE) tissue extracellular miRNA induced pluripotent stem (iPS) cells Ligo-miR microarrays Next Generation Sequencing

Editors and affiliations

  • Sweta Rani
    • 1
  1. 1.Department of ScienceWaterford Institute of TechnologyWaterfordIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6524-3
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6522-9
  • Online ISBN 978-1-4939-6524-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book