Editors:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1501)
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Table of contents (17 protocols)
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Front Matter
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In Vivo Models
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Front Matter
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In Vitro 2D & 3D-Models
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Front Matter
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Translational Applications
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Front Matter
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About this book
This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists, and those with an interest in molecular mechanisms underlying breast cancer.
Keywords
- genetically modified mouse models
- pubertal mammary gland phenotypes
- 2D and 3D-model culture systems
- stem cells
- mammary stem cell isolation
- human breast cancer
Editors and Affiliations
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School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Ireland
Finian Martin
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Institute of Cancer Sciences College of MVLS University of Glasgow, Glasgow, United Kingdom
Torsten Stein
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Division of Oncology-Pathology, Lund University Cancer Center/Medicon Village, Lund, Sweden
Jillian Howlin
Bibliographic Information
Book Title: Mammary Gland Development
Book Subtitle: Methods and Protocols
Editors: Finian Martin, Torsten Stein, Jillian Howlin
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6475-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6473-4Published: 29 October 2016
Softcover ISBN: 978-1-4939-8212-7Published: 10 July 2018
eBook ISBN: 978-1-4939-6475-8Published: 28 October 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 358
Number of Illustrations: 30 b/w illustrations, 32 illustrations in colour
Topics: Biomedical Research