Book Volume 1498 2017

In Vitro Mutagenesis

Methods and Protocols

Editors:

ISBN: 978-1-4939-6470-3 (Print) 978-1-4939-6472-7 (Online)

Table of contents (34 protocols)

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  1. In Vitro Transposon Mutagenesis Methods in Diverse Prokaryotic Systems

    1. Front Matter

      Pages 255-255

    2. No Access

      Protocol

      Pages 321-335

      Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis

  2. Site-Directed Mutagenesis: PCR and DNA Polymerase-Based Methods

    1. Front Matter

      Pages 337-337

    2. No Access

      Protocol

      Pages 339-347

      Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method

    3. No Access

      Protocol

      Pages 349-357

      Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis

    4. No Access

      Protocol

      Pages 359-366

      Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly

    5. No Access

      Protocol

      Pages 367-374

      Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method

    6. No Access

      Protocol

      Pages 375-383

      An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology

    7. No Access

      Protocol

      Pages 385-396

      Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function

  3. In Vitro Mutagenesis for Studies of Protein Structure and Function

    1. Front Matter

      Pages 397-397

    2. No Access

      Protocol

      Pages 399-419

      Step-By-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL

    3. No Access

      Protocol

      Pages 421-438

      Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein–Cofactor and Protein–Protein Functional Relationships

    4. No Access

      Protocol

      Pages 439-460

      Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions

    5. No Access

      Protocol

      Pages 461-480

      Use of Random and Site-Directed Mutagenesis to Probe Protein Structure–Function Relationships: Applied Techniques in the Study of Helicobacter pylori

  4. Random Mutagenesis: Novel PCR-Based Methods

    1. Front Matter

      Pages 481-481

    2. No Access

      Protocol

      Pages 483-490

      Novel Random Mutagenesis Method for Directed Evolution

    3. No Access

      Protocol

      Pages 491-495

      Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent

    4. No Access

      Protocol

      Pages 497-506

      Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR)

  5. Back Matter

    Pages 507-511

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