Book Volume 1498 2017

In Vitro Mutagenesis

Methods and Protocols

Editors:

ISBN: 978-1-4939-6470-3 (Print) 978-1-4939-6472-7 (Online)

Table of contents (34 protocols)

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  1. Front Matter

    Pages i-xv

  2. Gene and Genome-Editing Methods Part I

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-21

      Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells

    3. No Access

      Protocol

      Pages 23-31

      Mutagenesis and Genome Engineering of Epstein–Barr Virus in Cultured Human Cells by CRISPR/Cas9

    4. No Access

      Protocol

      Pages 33-40

      Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice

    5. No Access

      Protocol

      Pages 41-56

      All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells

    6. No Access

      Protocol

      Pages 57-78

      CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium

    7. No Access

      Protocol

      Pages 79-103

      Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii

  3. Gene and Genome-Editing Methods Part II

    1. Front Matter

      Pages 105-105

    2. No Access

      Protocol

      Pages 107-120

      Generation of Stable Knockout Mammalian Cells by TALEN-Mediated Locus-Specific Gene Editing

    3. No Access

      Protocol

      Pages 121-133

      Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-cloned Technology

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      Protocol

      Pages 135-152

      Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes

    5. No Access

      Protocol

      Pages 153-162

      Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells

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      Protocol

      Pages 163-177

      Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis

  4. Bioinformatics Approaches for Identifying and Analyzing Mutagenesis Targets

    1. Front Matter

      Pages 179-179

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      Protocol

      Pages 181-190

      In Silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein–Cofactor and Protein–Protein Functional Relationships

    3. No Access

      Protocol

      Pages 191-197

      In Silico Prediction of Deleteriousness for Nonsynonymous and Splice-Altering Single Nucleotide Variants in the Human Genome

    4. No Access

      Protocol

      Pages 199-226

      In Silico Methods for Analyzing Mutagenesis Targets

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      Protocol

      Pages 227-242

      Methods for Detecting Critical Residues in Proteins

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      Protocol

      Pages 243-253

      A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants

  5. In Vitro Transposon Mutagenesis Methods in Diverse Prokaryotic Systems

    1. Front Matter

      Pages 255-255

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      Protocol

      Pages 257-271

      Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea

    3. No Access

      Protocol

      Pages 273-293

      Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors

    4. No Access

      Protocol

      Pages 295-308

      PERMutation Using Transposase Engineering (PERMUTE): A Simple Approach for Constructing Circularly Permuted Protein Libraries

    5. No Access

      Protocol

      Pages 309-320

      Transposon Insertion Mutagenesis for Archaeal Gene Discovery

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