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Genotyping

Methods and Protocols

  • Book
  • © 2017

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1492)

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About this book

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.

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Keywords

Table of contents (17 protocols)

Editors and Affiliations

  • Leiden Genome Technology Center Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands

    Stefan J. White

  • Department of Genome Sciences, University of Washington School of Medicine, Seattle, USA

    Stuart Cantsilieris

Bibliographic Information

  • Book Title: Genotyping

  • Book Subtitle: Methods and Protocols

  • Editors: Stefan J. White, Stuart Cantsilieris

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6442-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6440-6Published: 08 November 2016

  • Softcover ISBN: 978-1-4939-8201-1Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6442-0Published: 07 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 254

  • Number of Illustrations: 10 b/w illustrations, 55 illustrations in colour

  • Topics: Human Genetics

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