Genotyping

Methods and Protocols

  • Stefan J. White
  • Stuart Cantsilieris

Part of the Methods in Molecular Biology book series (MIMB, volume 1492)

Table of contents

  1. Front Matter
    Pages i-x
  2. Angelika Heissl, Barbara Arbeithuber, Irene Tiemann-Boege
    Pages 29-57
  3. Tomasz Krzywkowski, Thomas Hauling, Mats Nilsson
    Pages 59-76
  4. Justine A. Ellis, Benjamin Ong
    Pages 77-94
  5. Stuart Cantsilieris, Holly A. Stessman, Jay Shendure, Evan E. Eichler
    Pages 95-106
  6. Jess Tyson, John A. L. Armour
    Pages 155-165
  7. Rolf H. A. M Vossen, Stefan J. White
    Pages 167-177
  8. Rolf H. A. M. Vossen, Henk P. J. Buermans
    Pages 179-184
  9. Quint P. Hottentot, M. van Min, E. Splinter, Stefan J. White
    Pages 185-196
  10. Kingsley K. Amoako, Matthew C. Thomas, Timothy W. Janzen, Noriko Goji
    Pages 203-220
  11. Beth A. Rowan, Danelle K. Seymour, Eunyoung Chae, Derek S. Lundberg, Detlef Weigel
    Pages 221-242
  12. Johan T. den Dunnen
    Pages 243-251
  13. Back Matter
    Pages 253-254

About this book

Introduction

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.

Keywords

DNA Taqman-based assays RNA molecules Molecular Inversion Probes copy number variation (CNV) Pulsed Field Gel Electrophoresis (PFGE) Paralogue Ratio Test (PRT) haplotype generation PacBio sequencing

Editors and affiliations

  • Stefan J. White
    • 1
  • Stuart Cantsilieris
    • 2
  1. 1.Leiden Genome Technology Center Department of Human GeneticsLeiden University Medical CenterLeidenThe Netherlands
  2. 2.Department of Genome SciencesUniversity of Washington School of MedicineSeattleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6442-0
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6440-6
  • Online ISBN 978-1-4939-6442-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book