© 2017

Optical Tweezers

Methods and Protocols

  • Arne Gennerich


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1486)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Theory and Design of Optical Tweezers

    1. Front Matter
      Pages 1-1
    2. Matthias D. Koch, Joshua W. Shaevitz
      Pages 3-24
    3. Rafael S. Dutra, Nathan B. Viana, Paulo A. Maia Neto, H. Moysés Nussenzveig
      Pages 25-39
    4. Stephen R. Okoniewski, Ashley R. Carter, Thomas T. Perkins
      Pages 77-107
    5. Michael Bugiel, Anita Jannasch, Erik Schäffer
      Pages 109-136
    6. Anita Jannasch, Mohammad K. Abdosamadi, Avin Ramaiya, Suman De, Valentina Ferro, Aaron Sonnberger et al.
      Pages 137-155
    7. Zhanna Santybayeva, Francesco Pedaci
      Pages 157-181
    8. Iddo Heller, Niels Laurens, Daan Vorselen, Onno D. Broekmans, Andreas S. Biebricher, Graeme A. King et al.
      Pages 257-272
  3. Optical Tweezers-Based Manipulation and Analysis of DNA-DNA Interactions, Protein Unfolding/Refolding, and Motion/Force-Generation by Mechanoenzymes In Vitro

    1. Front Matter
      Pages 273-273
    2. Ineke Brouwer, Graeme A. King, Iddo Heller, Andreas S. Biebricher, Erwin J. G. Peterman, Gijs J. L. Wuite
      Pages 275-293
    3. Juan Carlos Cordova, Adrian O. Olivares, Matthew J. Lang
      Pages 317-341
    4. Shixin Liu, Sara Tafoya, Carlos Bustamante
      Pages 343-355
    5. Junyi Jiao, Aleksander A. Rebane, Lu Ma, Yongli Zhang
      Pages 357-390
    6. Anirban Chakraborty, Cong A. Meng, Steven M. Block
      Pages 391-409
    7. Marian Baclayon, Svenja-Marei Kalisch, Ed Hendel, Liedewij Laan, Julien Husson, E. Laura Munteanu et al.
      Pages 411-435

About this book


The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines. 


optical tweezers setups single-molecule fluorescence imaging force measurements quantitative force measurements optical traps

Editors and affiliations

  • Arne Gennerich
    • 1
  1. 1.Department of Anatomy and Structural Biology & Gruss-Lipper Biophotonics CenterAlbert Einstein College of MedicineBronxUSA

Bibliographic information

  • Book Title Optical Tweezers
  • Book Subtitle Methods and Protocols
  • Editors Arne Gennerich
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-6419-2
  • Softcover ISBN 978-1-4939-8194-6
  • eBook ISBN 978-1-4939-6421-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 555
  • Number of Illustrations 21 b/w illustrations, 133 illustrations in colour
  • Topics Biotechnology
  • Buy this book on publisher's site