© 2016

Wnt Signaling

Methods and Protocols

  • Quinn Barrett
  • Lawrence Lum


  • Includes cutting-edge methods and protocols for the study of Wnt signaling

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1481)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Xinxin Gao, Rami N. Hannoush
    Pages 1-9
  3. Rubina Tuladhar, Nageswari Yarravarapu, Lawrence Lum
    Pages 11-16
  4. Kathrin Glaeser, Michael Boutros, Julia Christina Gross
    Pages 17-28
  5. Annette S. Flozak, Anna P. Lam, Cara J. Gottardi
    Pages 49-60
  6. Kristin B. Runkle, Eric S. Witze
    Pages 61-68
  7. Lisa M. Galli, Tiffany Barnes, Laura W. Burrus
    Pages 69-80
  8. Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, Natascia Tiso
    Pages 81-94
  9. Li-shu Zhang, Lawrence Lum
    Pages 111-117
  10. Zhendong A. Zhong, Nicole J. Ethen, Bart O. Williams
    Pages 119-125
  11. Amy T. Ku, Qi Miao, Hoang Nguyen
    Pages 127-140
  12. Jarno Drost, Benedetta Artegiani, Hans Clevers
    Pages 141-159
  13. Ian J. Huggins, David Brafman, Karl Willert
    Pages 161-181
  14. Back Matter
    Pages 197-198

About this book


Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.


Regenerative medicine Cancer management Wnt transcriptional responses Tissue engineering Therapeutics Model organisms

Editors and affiliations

  • Quinn Barrett
    • 1
  • Lawrence Lum
    • 2
  1. 1.University of Texas Southwestern Medical CenterDallasUSA
  2. 2.Department of Cell BiologyUniversity of Texas Southwestern Medical CenterDallasUSA

Bibliographic information

  • Book Title Wnt Signaling
  • Book Subtitle Methods and Protocols
  • Editors Quinn Barrett
    Lawrence Lum
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-6391-1
  • Softcover ISBN 978-1-4939-8185-4
  • eBook ISBN 978-1-4939-6393-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 198
  • Number of Illustrations 13 b/w illustrations, 32 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site