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  • © 2017

Synthetic DNA

Methods and Protocols

Editors:

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  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1472)

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USD 89.00
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  • ISBN: 978-1-4939-6343-0
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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
  2. Computational Tools for Design and Assembly of Synthetic DNA

    1. Front Matter

      Pages 1-1
    2. Synthetic Gene Design Using Codon Optimization On-Line (COOL)

      • Kai Yu, Kok Siong Ang, Dong-Yup Lee
      Pages 13-34
  3. DNA Synthesis, Assembly, and Cloning

    1. Front Matter

      Pages 47-47
    2. BASIC: A Simple and Accurate Modular DNA Assembly Method

      • Marko Storch, Arturo Casini, Ben Mackrow, Tom Ellis, Geoff S. Baldwin
      Pages 79-91
    3. Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides

      • Cosimo Ducani, Björn Högberg
      Pages 93-103
    4. PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly

      • Maryia Trubitsyna, Chao-Kuo Liu, Alejandro Salinas, Alistair Elfick, Christopher E. French
      Pages 111-128
    5. Generation of DNA Constructs Using the Golden GATEway Cloning Method

      • Stephan Kirchmaier, Katharina Lust, Jochen Wittbrodt
      Pages 157-168
    6. Gene Deletion by Synthesis in Yeast

      • Jinsil Kim, Dong-Uk Kim, Kwang-Lae Hoe
      Pages 169-185
    7. Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)

      • Sunil Chandran, Elaine Shapland
      Pages 187-192
    8. Rapid Construction of Recombinant Plasmids by QuickStep-Cloning

      • Pawel Jajesniak, Tuck Seng Wong
      Pages 205-214
  4. Reducing Error in Synthetic DNA

    1. Front Matter

      Pages 215-215

About this book

This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology.  Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.

Keywords

  • synthetic oligonucleotide sequences
  • COOL program
  • DNA sequences
  • BASIC
  • chemically synthesized DNA
  • oligonucleotide synthesis

Editors and Affiliations

  • Applied Research Laboratories, The University of Texas at Austin, AUSTIN, USA

    Randall A. Hughes

Bibliographic Information

  • Book Title: Synthetic DNA

  • Book Subtitle: Methods and Protocols

  • Editors: Randall A. Hughes

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6343-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6341-6

  • Softcover ISBN: 978-1-4939-8170-0

  • eBook ISBN: 978-1-4939-6343-0

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 248

  • Number of Illustrations: 14 b/w illustrations, 60 illustrations in colour

  • Topics: Medical Genetics

Buying options

eBook
USD 89.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6343-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 119.99
Price excludes VAT (USA)
Hardcover Book
USD 159.99
Price excludes VAT (USA)