© 2017

Synthetic DNA

Methods and Protocols

  • Randall A. Hughes


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1472)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Computational Tools for Design and Assembly of Synthetic DNA

    1. Front Matter
      Pages 1-1
    2. Kai Yu, Kok Siong Ang, Dong-Yup Lee
      Pages 13-34
  3. DNA Synthesis, Assembly, and Cloning

    1. Front Matter
      Pages 47-47
    2. Marko Storch, Arturo Casini, Ben Mackrow, Tom Ellis, Geoff S. Baldwin
      Pages 79-91
    3. Cosimo Ducani, Björn Högberg
      Pages 93-103
    4. Maryia Trubitsyna, Chao-Kuo Liu, Alejandro Salinas, Alistair Elfick, Christopher E. French
      Pages 111-128
    5. Stephan Kirchmaier, Katharina Lust, Jochen Wittbrodt
      Pages 157-168
    6. Jinsil Kim, Dong-Uk Kim, Kwang-Lae Hoe
      Pages 169-185
    7. Sunil Chandran, Elaine Shapland
      Pages 187-192
    8. Pawel Jajesniak, Tuck Seng Wong
      Pages 205-214
  4. Reducing Error in Synthetic DNA

    1. Front Matter
      Pages 215-215

About this book


This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology.  Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.


synthetic oligonucleotide sequences COOL program DNA sequences BASIC chemically synthesized DNA oligonucleotide synthesis

Editors and affiliations

  • Randall A. Hughes
    • 1
  1. 1.Applied Research LaboratoriesThe University of Texas at AustinAUSTINUSA

Bibliographic information

  • Book Title Synthetic DNA
  • Book Subtitle Methods and Protocols
  • Editors Randall A. Hughes
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-6341-6
  • Softcover ISBN 978-1-4939-8170-0
  • eBook ISBN 978-1-4939-6343-0
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIII, 248
  • Number of Illustrations 14 b/w illustrations, 60 illustrations in colour
  • Topics Human Genetics
  • Buy this book on publisher's site