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  • © 2016

Chromosome and Genomic Engineering in Plants

Methods and Protocols

Editors:

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  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1469)

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USD 109.00
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  • ISBN: 978-1-4939-4931-1
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USD 139.00
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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences

    • Nathaniel Graham, Nathan Swyers, Jon Cody, Morgan McCaw, Changzeng Zhao, James A. Birchler
    Pages 1-13
  3. Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase

    • Ruyu Li, Zhiguo Han, Lili Hou, Gurminder Kaur, Qian Yin, David W. Ow
    Pages 15-30
  4. One-Step Generation of Chromosomal Rearrangements in Rice

    • Minoru Murata, Asaka Kanatani, Kazunari Kashihara
    Pages 63-76
  5. Gametocidal System for Dissecting Wheat Chromosomes

    • Hisashi Tsujimoto
    Pages 101-109
  6. Targeted Mutagenesis in Rice Using TALENs and the CRISPR/Cas9 System

    • Masaki Endo, Ayako Nishizawa-Yokoi, Seiichi Toki
    Pages 123-135
  7. Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination

    • Ayako Nishizawa-Yokoi, Hiroaki Saika, Seiichi Toki
    Pages 137-146
  8. Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells

    • Takehito Kobayashi, Yusuke Yagi, Takahiro Nakamura
    Pages 147-155
  9. Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes

    • Petr Cápal, Jan Vrána, Marie Kubaláková, Takashi R. Endo, Jaroslav Doležel
    Pages 157-170
  10. Image Analysis of DNA Fiber and Nucleus in Plants

    • Nobuko Ohmido, Toshiyuki Wako, Seiji Kato, Kiichi Fukui
    Pages 171-180
  11. Detection of Transgenes on DNA Fibers

    • Fukashi Shibata
    Pages 181-188
  12. Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements

    • Satoru Fujimoto, Sachihiro Matsunaga, Minoru Murata
    Pages 207-216
  13. Back Matter

    Pages 217-218

About this book

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.

 

Keywords

  • plant genetics & genomics
  • DNA insertion
  • plant chromosome vector
  • plant artificial chromosomes
  • Cre/LoxP
  • Ac/DS system
  • genome editing

Editors and Affiliations

  • Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan

    Minoru Murata

Bibliographic Information

  • Book Title: Chromosome and Genomic Engineering in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Minoru Murata

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-4931-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-4929-8

  • Softcover ISBN: 978-1-4939-8163-2

  • eBook ISBN: 978-1-4939-4931-1

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 218

  • Number of Illustrations: 24 b/w illustrations, 38 illustrations in colour

  • Topics: Plant Science

Buying options

eBook
USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-4931-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 139.00
Price excludes VAT (USA)
Hardcover Book
USD 159.99
Price excludes VAT (USA)