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Methods and Protocols

  • Book
  • © 2016


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1454)

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About this book

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.


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Table of contents (16 protocols)

Editors and Affiliations

  • Albert Einstein College of Medicine, Yeshiva University, Bronx, USA

    Peter Satir

  • Department of Biology, University of Copenhagen, Copenhagen, Denmark

    Søren Tvorup Christensen

Bibliographic Information

  • Book Title: Cilia

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Satir, Søren Tvorup Christensen

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3787-5Published: 12 August 2016

  • Softcover ISBN: 978-1-4939-8139-7Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3789-9Published: 11 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 259

  • Number of Illustrations: 15 b/w illustrations, 39 illustrations in colour

  • Topics: Cell Biology

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