© 2016


Methods and Protocols

  • Koichi Kawakami
  • E. Elizabeth Patton
  • Michael Orger
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1451)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Genetics and Genomics

    1. Front Matter
      Pages 1-1
    2. Colleen A. Brady, Andrew J. Rennekamp, Randall T. Peterson
      Pages 3-16
    3. Alvin C. H. Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker
      Pages 17-30
    4. Yavor Hadzhiev, Irene Miguel-Escalada, Darius Balciunas, Ferenc Müller
      Pages 81-91
    5. Hideyuki Tanabe, Masahide Seki, Mari Itoh, Ailani Deepak, Pradeep Lal, Terumi Horiuchi et al.
      Pages 93-106
    6. Minori Shinya
      Pages 107-118
  3. Disease Models and Mechanism

    1. Front Matter
      Pages 119-119
    2. Genevieve C. Kendall, James F. Amatruda
      Pages 121-142
    3. Sonia Wojciechowska, Zhiqiang Zeng, James A. Lister, Craig J. Ceol, E. Elizabeth Patton
      Pages 143-153
    4. Claudia Tulotta, Shuning He, Lanpeng Chen, Arwin Groenewoud, Wietske van der Ent, Annemarie H. Meijer et al.
      Pages 155-169
    5. Vinothkumar Rajan, Graham Dellaire, Jason N. Berman
      Pages 171-189
    6. Virginie Esain, Mauricio Cortes, Trista E. North
      Pages 191-206
    7. Molly A. Matty, Stefan H. Oehlers, David M. Tobin
      Pages 207-223
    8. Erin M. Zeituni, Steven A. Farber
      Pages 237-255
  4. Neuroscience

    1. Front Matter
      Pages 257-257

About this book


This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. Zebrafish: Methods and Protocols, Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish,  applications of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection and host-pathogen interactions, metabolism and transport of lipids, and  the structure and function of neural circuits and their role in generating behavior. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a useful complement to the first book for new and experienced zebrafish researcher alike. 


zebrafish mutagenesis genome editing PhiC31 system transgenesis zebrafish hematopoietic system

Editors and affiliations

  • Koichi Kawakami
    • 1
  • E. Elizabeth Patton
    • 2
  • Michael Orger
    • 3
  1. 1.Division of Molecular and Developmental BiologyNational Institute of GeneticsMishimaJapan
  2. 2.Institute of Genetics and Molecular MedicineMRC Human Genetics Unit & The University of Edinburgh Cancer Research UK CentreEdinburghUnited Kingdom
  3. 3.Champalimaud Centre for the UnknownChampalimaud Neuroscience Programme Champalimaud Centre for the UnknownLisbonPortugal

Bibliographic information

  • Book Title Zebrafish
  • Book Subtitle Methods and Protocols
  • Editors Koichi Kawakami
    E. Elizabeth Patton
    Michael Orger
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3769-1
  • Softcover ISBN 978-1-4939-8134-2
  • eBook ISBN 978-1-4939-3771-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XII, 373
  • Number of Illustrations 15 b/w illustrations, 64 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site