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  • © 2016

Heterologous Expression of Membrane Proteins

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of recombinant integral membrane

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1432)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
  2. Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

    • Ralf-Bernhardt Rues, Erik Henrich, Coilin Boland, Martin Caffrey, Frank Bernhard
    Pages 1-21
  3. Membrane Protein Production in the Yeast, S. cerevisiae

    • Stephanie P. Cartwright, Lina Mikaliunaite, Roslyn M. Bill
    Pages 23-35
  4. Membrane Protein Production in Escherichia coli: Protocols and Rules

    • Federica Angius, Oana Ilioaia, Marc Uzan, Bruno Miroux
    Pages 37-52
  5. Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach

    • Kiavash Mirzadeh, Stephen Toddo, Morten H. H. Nørholm, Daniel O. Daley
    Pages 53-61
  6. Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins

    • Juni Andréll, Patricia C. Edwards, Fan Zhang, Maria Daly, Christopher G. Tate
    Pages 63-78
  7. Membrane Protein Production in Lactococcus lactis for Functional Studies

    • Daphne Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty, François André et al.
    Pages 79-101
  8. Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells

    • Renato Mancini Astray, Sandra Fernanda Suárez-Patiño, Soraia Attie Calil Jorge
    Pages 103-118
  9. Expression of Eukaryotic Membrane Proteins in Pichia pastoris

    • Lucie Hartmann, Valérie Kugler, Renaud Wagner
    Pages 143-162
  10. Integral Membrane Protein Expression in Saccharomyces cerevisiae

    • Rebba C. Boswell-Casteel, Jennifer M. Johnson, Robert M. Stroud, Franklin A. Hays
    Pages 163-186
  11. High-Throughput Baculovirus Expression System for Membrane Protein Production

    • Ravi C. Kalathur, Marinela Panganiban, Renato Bruni
    Pages 187-202
  12. Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies

    • Sarika Chaudhary, Sukanya Saha, Sobrahani Thamminana, Robert M. Stroud
    Pages 203-221
  13. Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies

    • Rajendra Boggavarapu, Stephan Hirschi, Daniel Harder, Marcel Meury, Zöhre Ucurum, Marc J. Bergeron et al.
    Pages 223-242
  14. Membrane Protein Solubilization and Composition of Protein Detergent Complexes

    • Katia Duquesne, Valérie Prima, James N. Sturgis
    Pages 243-260
  15. Detergent-Free Membrane Protein Purification

    • Alice J. Rothnie
    Pages 261-267

About this book

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

Keywords

  • Integral membrane proteins
  • Recombinant IMPs
  • G protein-coupled receptors
  • GPCRs
  • Purification
  • Pharmaceuticals
  • Drug targets

Editors and Affiliations

  • Université Côte d’Azur CNRS, IPMC, Valbonne, France

    Isabelle Mus-Veteau

Bibliographic Information

  • Book Title: Heterologous Expression of Membrane Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Isabelle Mus-Veteau

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3637-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3635-9Published: 03 August 2016

  • Softcover ISBN: 978-1-4939-8102-1Published: 30 May 2018

  • eBook ISBN: 978-1-4939-3637-3Published: 02 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 322

  • Number of Illustrations: 27 b/w illustrations, 39 illustrations in colour

  • Topics: Protein Science, Membrane Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access