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Angiogenesis Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Stewart G. Martin

    1. Nottingham, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Peter W. Hewett

    1. UNIV BIRMINGHAM/MEDICAL SCHOOL, BIRMINGHAM B15 2TT, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1430)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
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  2. Microscopic Assessment

    1. Front Matter

      Pages 1-1
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    2. Assessing Tumor Angiogenesis in Histological Samples

      • Jia-Min Pang, Nicholas Jene, Stephen B. Fox
      Pages 3-33

    3. Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis

      • Daniel J. Royston, Steven Clasper, David G. Jackson
      Pages 35-48

    4. Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis

      • Narmeen S. Ahmad, Stewart G. Martin, Sarah J. Storr
      Pages 49-57

  3. Cell Isolation Techniques

    1. Front Matter

      Pages 59-59
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    2. Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels

      • Peter W. Hewett
      Pages 61-76

    3. Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells

      • Zerina Lokmic
      Pages 77-90

    4. Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells

      • Jessal J. Patel, Salil Srivastava, Richard C. M. Siow
      Pages 91-105

    5. Isolation and Transfection of Primary Culture Bovine Retinal Pericytes

      • Vincent A. Primo, Joseph F. Arboleda-Velasquez
      Pages 107-117

  4. In Vitro Techniques

    1. Front Matter

      Pages 119-119
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    2. In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis

      • Suzanne A. Eccles, William Court, Lisa Patterson
      Pages 121-147

    3. Tube-Forming Assays

      • Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles, Roy Bicknell
      Pages 149-157

    4. In Vitro Coculture Assays of Angiogenesis

      • Mark Richards, Harry Mellor
      Pages 159-166

    5. Spheroid-Based In Vitro Angiogenesis Model

      • Larissa Pfisterer, Thomas Korff
      Pages 167-177

    6. Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis

      • Fatemeh Sharifpanah, Heinrich Sauer
      Pages 179-189

    7. Human Arterial Ring Angiogenesis Assay

      • Giorgio Seano, Luca Primo
      Pages 191-203

    8. A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro

      • Nina Zippel, Yindi Ding, Ingrid Fleming
      Pages 205-219

    9. Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis

      • Anthony R. Sheets, Jennifer T. Durham, Ira M. Herman
      Pages 221-229

    10. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature

      • Lynn M. Butler, Helen M. McGettrick, Gerard B. Nash
      Pages 231-248
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Keywords

About this book

In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols, Third Edition also guides readers through new protocols on chorioallantoic membrane models, corneal pocket assays to assess angio- and lymphangiogenesis, models of muscle angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and senescence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Angiogenesis Protocols, Third Edition provides not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Editors and Affiliations

  • Nottingham, United Kingdom

    Stewart G. Martin

  • UNIV BIRMINGHAM/MEDICAL SCHOOL, BIRMINGHAM B15 2TT, United Kingdom

    Peter W. Hewett

Bibliographic Information

  • Book Title: Angiogenesis Protocols

  • Editors: Stewart G. Martin, Peter W. Hewett

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3628-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3626-7Published: 13 May 2016

  • Softcover ISBN: 978-1-4939-8099-4Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3628-1Published: 12 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 404

  • Number of Illustrations: 20 b/w illustrations, 42 illustrations in colour

  • Topics: Cell Biology

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