Editors:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implelementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1412)
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Table of contents (28 protocols)
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Front Matter
About this book
This
volume encompasses all major methodologies to interrogate endocannabinoid
systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest
towards the manifold activities of eCBs, this book discusses the chemical,
biochemical, and molecular biological assays, and activity of distinct elements
of the ECS. These include membrane, nuclear receptors, biosynthetic and
hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in
the highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocols
is a valuable resource and will help chemists, drug designers, biochemists,
molecular biologists, cell biologists, pharmacologists, and (electro)
physiologists navigate the mare magnum of endocannabinoid research.
Keywords
- Cannabis sativa
- endocannabinoid system
- N-arachidonoylethanolamine
- 2-arachidonoylglycerol
- membrane and nuclear receptors
- biosynthetic and hydrolytic enzymes
- membrane transporters
- oxidative enzymes
- drug design
- pharmacology
Editors and Affiliations
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Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy
Mauro Maccarrone
Bibliographic Information
Book Title: Endocannabinoid Signaling
Book Subtitle: Methods and Protocols
Editors: Mauro Maccarrone
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3539-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3537-6Published: 01 June 2016
Softcover ISBN: 978-1-4939-8071-0Published: 26 May 2018
eBook ISBN: 978-1-4939-3539-0Published: 31 May 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 285
Number of Illustrations: 49 b/w illustrations, 26 illustrations in colour
Topics: Neuroscience