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Animal Coronaviruses

  • Book
  • © 2016


  • Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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About this book

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

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Table of contents (19 protocols)

  1. Introduction

  2. Conventional Technologies

  3. Reverse Genetics

  4. Molecular Diagnostics


“This book is unique in presenting current technologies available for detection of animal coronaviruses and research especially for diagnostic methodologies. The intention is to provide specific protocols and methods for individual coronaviruses, both conventional approaches and state-of-the art technologies. … The primary audience is diagnosticians, interns, and residents who work in the laboratory, but graduate students and researchers will benefit as well. … The book is essential for researchers and diagnosticians in veterinary virology.” (Dongwan Yoo, Doody's Book Reviews, June, 2016)

Editors and Affiliations

  • Animal Disease Diagnostic Lab, Ohio Department of Agriculture, Reynoldsburg, USA

    Leyi Wang

Bibliographic Information

  • Book Title: Animal Coronaviruses

  • Editors: Leyi Wang

  • Series Title: Springer Protocols Handbooks

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3412-6Published: 23 March 2016

  • Softcover ISBN: 978-1-4939-8044-4Published: 19 April 2018

  • eBook ISBN: 978-1-4939-3414-0Published: 22 March 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 218

  • Number of Illustrations: 10 b/w illustrations, 15 illustrations in colour

  • Topics: Infectious Diseases

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