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Nucleic Acid Aptamers

Selection, Characterization, and Application

  • Günter Mayer

Part of the Methods in Molecular Biology book series (MIMB, volume 1380)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Selection

    1. Front Matter
      Pages 1-1
    2. Yi Long, Franziska Pfeiffer, Günter Mayer, Tine Daa Schrøder, Veli Cengiz Özalp, Lars Folke Olsen
      Pages 3-19
    3. Katharina Berg, Eileen Magbanua, Ulrich Hahn
      Pages 21-32
    4. Silvia Catuogno, Carla Lucia Esposito, Vittorio de Franciscis
      Pages 33-46
    5. Fabian Tolle, Günter Mayer
      Pages 77-84
  3. Characterization

    1. Front Matter
      Pages 97-97
    2. Dennis Breitsprecher, Nina Schlinck, David Witte, Stefan Duhr, Philipp Baaske, Thomas Schubert
      Pages 99-111
    3. Isis C. Nascimento, Arthur A. Nery, Vinícius Bassaneze, José E. Krieger, Henning Ulrich
      Pages 127-134
    4. Ioanna Théodorou, Nam Nguyen Quang, Karine Gombert, Benoit Thézé, Benoit Lelandais, Frédéric Ducongé
      Pages 135-150
  4. Application

    1. Front Matter
      Pages 161-161
    2. Alessandro Pinto, Pedro Nadal Polo, Miriam Jauest Rubio, Marketa Svobodova, Teresa Mairal Lerga, Ciara K. O’Sullivan
      Pages 171-177
    3. Jens Müller, Tobias Becher, Günter Mayer, Bernd Pötzsch
      Pages 179-189
    4. Sarah Shigdar, Li Lv, Lifen Wang, Wei Duan
      Pages 191-196
    5. Maria Angela Gomes de Castro, Burkhard Rammner, Felipe Opazo
      Pages 197-210
    6. Michele Felletti, Benedikt Klauser, Jörg S. Hartig
      Pages 225-239
  5. Back Matter
    Pages 241-242

About this book

Introduction

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

Keywords

Aptamer generation process Aptamer-target interactions Biophysical properties Cell surfaces Nucleic acids Small molecules

Editors and affiliations

  • Günter Mayer
    • 1
  1. 1.University of BonnBonnGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3197-2
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3196-5
  • Online ISBN 978-1-4939-3197-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site