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  • © 2016

In Vitro Embryogenesis in Higher Plants

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material:

Part of the book series: Methods in Molecular Biology (MIMB, volume 1359)

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  • ISBN: 978-1-4939-3061-6
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Softcover Book USD 179.99
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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xviii
  2. Reviews on General Topics

    1. Front Matter

      Pages 1-1
    2. A Comparison of In Vitro and In Vivo Asexual Embryogenesis

      • Melanie L. Hand, Sacco de Vries, Anna M. G. Koltunow
      Pages 3-23
    3. Recent Advances on Genetic and Physiological Bases of In Vitro Somatic Embryo Formation

      • Maria Maddalena Altamura, Federica Della Rovere, Laura Fattorini, Simone D’Angeli, Giuseppina Falasca
      Pages 47-85
    4. Do Mitochondria Play a Central Role in Stress-Induced Somatic Embryogenesis?

      • Birgit Arnholdt-Schmitt, Carla Ragonezi, Hélia Cardoso
      Pages 87-100
    5. Dying with Style: Death Decision in Plant Embryogenesis

      • Shuanglong Huang, Mohamed M. Mira, Claudio Stasolla
      Pages 101-115
    6. Somatic Embryogenesis in Broad-Leaf Woody Plants: What We Can Learn from Proteomics

      • Sandra I. Correia, Ana C. Alves, Paula Veríssimo, Jorge M. Canhoto
      Pages 117-129
    7. Advances in Conifer Somatic Embryogenesis Since Year 2000

      • Krystyna Klimaszewska, Catherine Hargreaves, Marie-Anne Lelu-Walter, Jean-François Trontin
      Pages 131-166
    8. Molecular Aspects of Conifer Zygotic and Somatic Embryo Development: A Review of Genome-Wide Approaches and Recent Insights

      • Jean-François Trontin, Krystyna Klimaszewska, Alexandre Morel, Catherine Hargreaves, Marie-Anne Lelu-Walter
      Pages 167-207
    9. Androgenesis in Solanaceae

      • Jose M. Seguí-Simarro
      Pages 209-244
    10. Bioreactors for Plant Embryogenesis and Beyond

      • Liwen Fei, Pamela Weathers
      Pages 245-259
  3. Protocols of Somatic Embryogenesis in Selected Important Horticultural Plants

    1. Front Matter

      Pages 261-261
    2. Somatic Embryogenesis and Genetic Modification of Vitis

      • Sadanand A. Dhekney, Zhijian T. Li, Trudi N. L. Grant, Dennis J. Gray
      Pages 263-277
    3. Somatic Embryogenesis in Peach-Palm (Bactris gasipaes) Using Different Explant Sources

      • Douglas A. Steinmacher, Angelo Schuabb Heringer, Víctor M. Jiménez, Marguerite G. G. Quoirin, Miguel P. Guerra
      Pages 279-288
    4. Somatic Embryogenesis: Still a Relevant Technique in Citrus Improvement

      • Ahmad A. Omar, Manjul Dutt, Frederick G. Gmitter, Jude W. Grosser
      Pages 289-327
    5. Somatic Embryogenesis Induction and Plant Regeneration in Strawberry Tree (Arbutus unedo L.)

      • João F. Martins, Sandra I. Correia, Jorge M. Canhoto
      Pages 329-339
    6. Somatic Embryogenesis in Crocus sativus L.

      • Basar Sevindik, Yesim Yalcin Mendi
      Pages 351-357
    7. Somatic Embryogenesis in Lisianthus (Eustoma russellianum Griseb.)

      • Barbara Ruffoni, Laura Bassolino
      Pages 359-370

About this book

This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.


  • Embryogenesis
  • Gametic
  • Horticulture
  • In Vitro Embryo Induction
  • In Vivo Asexual Embryogenesis
  • Microspore
  • Plant Regeneration
  • Somatic
  • Zygotic

Editors and Affiliations

  • Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  • National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

About the editors

Maria Antonietta Germana' Dipartimento DEMETRA Università degli Studi di Palermo Viale Delle Scienze, 11 90128 Palermo -Italy e-mail: Tel. +39-09123896094 Fax: +39-09123860813 Cell. +39 388 0400044 Cell. +39 320 8397040

Maurizio Lambardi, CNR-IVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, Polo Scientifico, via Madonna del Piano n. 10, 50019 Sesto Fiorentino (Firenze), Italy
Email: Tel: +39-055-5225685

Bibliographic Information

  • Book Title: In Vitro Embryogenesis in Higher Plants

  • Editors: Maria Antonietta Germana, Maurizio Lambardi

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3060-9Published: 02 December 2015

  • Softcover ISBN: 978-1-4939-7982-0Published: 29 March 2019

  • eBook ISBN: 978-1-4939-3061-6Published: 30 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 558

  • Number of Illustrations: 3 b/w illustrations, 66 illustrations in colour

  • Topics: Plant Science

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-3061-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)