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  • © 2016

Phospho-Proteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1355)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-3049-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.00
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
  2. Techniques for Tagging Phospho-Moieties

    1. Front Matter

      Pages 1-1
    2. Phosphopeptide Detection with Biotin-Labeled Phos-tag

      • Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
      Pages 17-29
  3. Labeling Techniques for Quantitative Phosphoproteomics

    1. Front Matter

      Pages 51-51
    2. Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics

      • Lei Cheng, Trairak Pisitkun, Mark A. Knepper, Jason D. Hoffert
      Pages 53-70
    3. Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles

      • Daniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, Sebastian Lourido
      Pages 71-84
  4. Phosphoprotein Analysis by Mass Spectrometry

    1. Front Matter

      Pages 211-211
    2. Variable Digestion Strategies for Phosphoproteomics Analysis

      • Humberto Gonczarowska-Jorge, Margherita Dell’Aica, Clarissa Dickhut, René P. Zahedi
      Pages 225-239

About this book

This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phospho-Proteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Phospho-Proteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Keywords

  • Ser residues
  • Thr residues
  • Tyr residues
  • kinase-substrate relationships
  • phospho flow cytometry
  • phosphopeptides
  • phosphoproteins
  • phosphorylated proteins
  • quantitative phosphoproteomics
  • tissue samples

Editors and Affiliations

  • University of Copenhagen, Center for Protein Research, Copenhagen Nm, Denmark

    Louise von Stechow

Bibliographic Information

  • Book Title: Phospho-Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Louise von Stechow

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3049-4

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3048-7

  • Softcover ISBN: 978-1-4939-5001-0

  • eBook ISBN: 978-1-4939-3049-4

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 364

  • Topics: Protein Biochemistry

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-3049-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.00
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)