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Whole Genome Amplification

Methods and Protocols

  • Thomas Kroneis

Part of the Methods in Molecular Biology book series (MIMB, volume 1347)

Table of contents

  1. Front Matter
    Pages i-x
  2. Zbigniew Tadeusz Czyz, Stefan Kirsch, Bernhard Polzer
    Pages 1-14
  3. Jeffrey Sabina, John H. Leamon
    Pages 15-41
  4. Roland Kirchner, Marianna Alunni-Fabbroni
    Pages 43-55
  5. Zbigniew Tadeusz Czyż, Christoph A. Klein
    Pages 69-86
  6. Takaaki Kojima, Bo Zhu, Hideo Nakano
    Pages 87-100
  7. Amin El-Heliebi, Shukun Chen, Thomas Kroneis
    Pages 119-128
  8. Zbigniew Tadeusz Czyz, Nikolas H. Stoecklein, Bernhard Polzer
    Pages 141-162
  9. Karina Meden Sørensen
    Pages 163-178
  10. Gloria Gonzales Fortes, Johanna L. A. Paijmans
    Pages 179-195
  11. Eftychia Dimitriadou, Masoud Zamani Esteki, Joris Robert Vermeesch
    Pages 197-219
  12. Christin Gasch, Klaus Pantel, Sabine Riethdorf
    Pages 221-232
  13. Thomas Kroneis, Shukun Chen, Amin El-Heliebi
    Pages 245-261
  14. Back Matter
    Pages 279-284

About this book

Introduction

This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods, and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-microdissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration, and will help researchers, both new and experienced, yield successful results. 

Keywords

Circulating Tumor Cells Comparative Genome Hybridization DNA Formalin-Fixed Paraffin Laser Microdissection Single-Cell WGA Techniques

Editors and affiliations

  • Thomas Kroneis
    • 1
  1. 1.Institute of Cell BiologyHistology & Embryology, Medical University of GrazGrazAustria

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2990-0
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2989-4
  • Online ISBN 978-1-4939-2990-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site