Protein Arginylation

Methods and Protocols

  • Anna S. Kashina

Part of the Methods in Molecular Biology book series (MIMB, volume 1337)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Mauricio R. Galiano, Marta E. Hallak
    Pages 49-58
  3. Junling Wang, Anna S. Kashina
    Pages 73-77
  4. Sougata Saha, Junling Wang, Anna S. Kashina
    Pages 79-82
  5. Anna S. Kashina, John R. Yates III
    Pages 93-104
  6. Anna S. Kashina, John R. Yates III
    Pages 105-107
  7. Anne M. Wagner, John B. Warner, Haviva E. Garrett, Christopher R. Walters, E. James Petersson
    Pages 109-127
  8. H. Alexander Ebhardt
    Pages 129-138
  9. Back Matter
    Pages 147-149

About this book

Introduction

This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Arginylation: Methods and Protocols would interest the emerging body of scientists involved in the studies of posttranslational arginylation, and the rapidly growing community of researchers working on a broad range of posttranslational modifications, the analysis of which often meets similar challenges and utilizes similar principles as posttranslational arginylation.

Keywords

Cellular Proteome Posttranslational Modifications Protein Arginylation Protein Preparations Regulators Whole-Cell Assays

Editors and affiliations

  • Anna S. Kashina
    • 1
  1. 1.Department of Animal BiologyUniversity of Pennsylvania School of Veterinary MedicinePhiladelphiaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2935-1
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2934-4
  • Online ISBN 978-1-4939-2935-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book