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Muscarinic Receptor: From Structure to Animal Models

  • Book
  • © 2016

Overview

Editors:
  1. Jaromir Myslivecek

    1. Institute of Physiology, Charles University, Prague, Czech Republic

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  2. Jan Jakubik

    1. Department of Neurochemistry, Acad. of Sciences of the Czech Rep. Institute of Physiology, Prague, Czech Republic

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 107)

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About this book

This volume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computer‑based approaches to study muscarinic receptors. Muscarinic Receptor: From Structure to Animal Models guides readers through binding techniques, protocols to investigate molecular properties of muscarinic receptors, protocols to study muscarinic receptors in the central nervous system using autoradiography and PET studies and protocols on animals with knock-out and knock-in muscarinic genes to study role of muscarinic receptors in physiology and behavior. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Muscarinic Receptor: From Structure to Animal Models aims to ensure successful results in the further study of this vital field.

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xii
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  2. Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors

    • Ryoji Suno, Hidetsugu Asada, Takuya Kobayashi
    Pages 1-13

  3. Homology Modeling and Docking Evaluation of Human Muscarinic Acetylcholine Receptors

    • Trayder Thomas, David K. Chalmers, Elizabeth Yuriev
    Pages 15-35

  4. Radioligand Binding at Muscarinic Receptors

    • Esam E. El-Fakahany, Jan Jakubik
    Pages 37-68

  5. Binding Method for Detection of Muscarinic Acetylcholine Receptors in Receptor’s Natural Environment

    • Ikunobu Muramatsu, Hatsumi Yoshiki, Kiyonao Sada, Junsuke Uwada, Takanobu Taniguchi, Takayoshi Masuoka et al.
    Pages 69-81

  6. Use of Antibodies in the Research on Muscarinic Receptor Subtypes

    • Wisuit Pradidarcheep, Martin C. Michel
    Pages 83-94

  7. Allosteric Modulation of Muscarinic Receptors

    • Jan Jakubik, Esam E. El-Fakahany
    Pages 95-130

  8. Subcellular and Synaptic Localization of Muscarinic Receptors in Neurons Using High-Resolution Electron Microscopic Preembedding Immunogold Technique

    • Véronique Bernard
    Pages 131-146

  9. Investigation of Muscarinic Receptors by Fluorescent Techniques

    • Cornelius Krasel, Andreas Rinne, Moritz Bünemann
    Pages 147-158

  10. Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System

    • Vladimir Farar, Jaromir Myslivecek
    Pages 159-180

  11. Imaging of Muscarinic Receptors in the Central Nervous System

    • Hideo Tsukada, Shingo Nishiyama, Kazuhiro Takahashi
    Pages 181-203

  12. Detection of Non-neuronal Acetylcholine

    • Ignaz Karl Wessler, Charles James Kirkpatrick
    Pages 205-220

  13. Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release

    • Glenda Alquicer, Vladimír Doležal, Esam E. El-Fakahany
    Pages 221-233

  14. Regulation of Heart Contractility by M2 and M3 Muscarinic Receptors: Functional Studies Using Muscarinic Receptor Knockout Mouse

    • Takio Kitazawa, Hiroki Teraoka, Nao Harada, Kenta Ochi, Tatsuro Nakamura, Koichi Asakawa et al.
    Pages 235-259

  15. Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion

    • Stephan Steidl, David Ian Wasserman, Charles D. Blaha, John Yeomans
    Pages 261-282

  16. Back Matter

    Pages 283-287
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Editors and Affiliations

  • Institute of Physiology, Charles University, Prague, Czech Republic

    Jaromir Myslivecek

  • Department of Neurochemistry, Acad. of Sciences of the Czech Rep. Institute of Physiology, Prague, Czech Republic

    Jan Jakubik

Bibliographic Information

  • Book Title: Muscarinic Receptor: From Structure to Animal Models

  • Editors: Jaromir Myslivecek, Jan Jakubik

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-2858-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2857-6Published: 05 August 2015

  • Softcover ISBN: 978-1-4939-4971-7Published: 01 November 2016

  • eBook ISBN: 978-1-4939-2858-3Published: 31 July 2015

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 287

  • Number of Illustrations: 35 b/w illustrations, 42 illustrations in colour

  • Topics: Neurosciences

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