Cell-Penetrating Peptides

Methods and Protocols

  • Ülo Langel

Part of the Methods in Molecular Biology book series (MIMB, volume 1324)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Margus Pooga, Ülo Langel
      Pages 3-28
    3. Edmond Dupont, Alain Prochiantz, Alain Joliot
      Pages 29-37
    4. Mattias Hällbrink, Mati Karelson
      Pages 39-58
    5. Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, Gajendra Pal Singh Raghava
      Pages 59-69
  3. Methods to Test Mechanisms of Cell-Penetrating Peptides

    1. Front Matter
      Pages 71-71
    2. Fatemeh Madani, Astrid Gräslund
      Pages 73-87
    3. Sandrine Sagan, Chérine Bechara, Fabienne Burlina
      Pages 107-121
    4. Samuel Schmidt, Rike Wallbrecher, Toin H. van Kuppevelt, Roland Brock
      Pages 123-131
    5. Julia Uusna, Kent Langel, Ülo Langel
      Pages 133-148
    6. Henrik Helmfors, Staffan Lindberg, Ülo Langel
      Pages 163-174
  4. Applications of Cpps In Vitro and In Vivo

    1. Front Matter
      Pages 175-175
    2. Ping Zhang, Lidija Covic, Athan Kuliopulos
      Pages 191-203
    3. Kalle Pärn, Elo Eriste, Ülo Langel
      Pages 223-245
    4. Lin He, Peter D. Watson, Arwyn T. Jones
      Pages 247-259

About this book


Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one  briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of “naked” CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 Concise and easy-to-use, Cell-Penetrating Peptides: Methods and Protocols, Second Edition hopes to raise relevant questions for further development. 


CPP methods cargo complexes conjugated cargos electron microscopy endocytotic pathways internalization machinery membrane bound proteins/glycoproteins naked CPPs phospholipid membranes

Editors and affiliations

  • Ülo Langel
    • 1
  1. 1.Department of NeurochemistryStockholm UniversityStockholmSweden

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2806-4
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2805-7
  • Online ISBN 978-1-4939-2806-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book