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  • © 2015

Plant Mitochondria

Methods and Protocols

  • Includes cutting-edge methods and protocols in plant cell biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1305)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xxiii
  2. In Vitro RNA Uptake Studies in Plant Mitochondria

    • Szymon Kubiszewski-Jakubiak, Cyrille Megel, Elodie Ubrig, Thalia Salinas, Anne-Marie Duchêne, Laurence Maréchal-Drouard
    Pages 45-60
  3. In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria

    • Owen Duncan, Chris Carrie, Yan Wang, Monika W. Murcha
    Pages 61-81
  4. Plant Mitochondrial Proteomics

    • Nicolas L. Taylor, A. Harvey Millar
    Pages 83-106
  5. Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites

    • Markus Hartl, Ann-Christine König, Iris Finkemeier
    Pages 107-121
  6. A Flowchart to Analyze Protease Activity in Plant Mitochondria

    • Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, Elzbieta Glaser
    Pages 123-130
  7. Activity Measurements of Mitochondrial Enzymes in Native Gels

    • Peter Schertl, Hans-Peter Braun
    Pages 131-138
  8. Activity Assay for Plant Mitochondrial Enzymes

    • Shaobai Huang, Chun Pong Lee, A. Harvey Millar
    Pages 139-149
  9. Analysis of Type II NAD(P)H Dehydrogenases

    • Kathleen L. Soole, Chevaun A. Smith
    Pages 151-164
  10. Assessment of Respiration in Isolated Plant Mitochondria Using Clark-Type Electrodes

    • Richard P. Jacoby, A. Harvey Millar, Nicolas L. Taylor
    Pages 165-185
  11. Micro-Respiratory Measurements in Plants

    • Yun Shin Sew, A. Harvey Millar, Elke Stroeher
    Pages 187-196
  12. Improvements to Define Mitochondrial Metabolomics Using Nonaqueous Fractionation

    • Richard Fly, James Lloyd, Stephan Krueger, Alisdair Fernie, Margaretha J. van der Merwe
    Pages 197-210
  13. Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana

    • Theresa J. Reape, Joanna Kacprzyk, Niall Brogan, Lee Sweetlove, Paul F. McCabe
    Pages 211-221
  14. Imaging and Analysis of Mitochondrial Dynamics in Living Cells

    • Sanjaya B. Ekanayake, Amr M. El Zawily, Gaël Paszkiewicz, Aurélia Rolland, David C. Logan
    Pages 223-240
  15. Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors

    • Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer et al.
    Pages 241-252
  16. In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide

    • Marina Cvetkovska, Greg C. Vanlerberghe
    Pages 253-261

About this book

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria’s unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

Editors and Affiliations

  • La Trobe University, Bundoora, Australia

    James Whelan

  • The University of Western Australia, Crawley, Australia

    Monika W. Murcha

Bibliographic Information

  • Book Title: Plant Mitochondria

  • Book Subtitle: Methods and Protocols

  • Editors: James Whelan, Monika W. Murcha

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2639-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2638-1Published: 25 April 2015

  • Softcover ISBN: 978-1-4939-4906-9Published: 26 June 2017

  • eBook ISBN: 978-1-4939-2639-8Published: 24 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXIII, 303

  • Number of Illustrations: 12 b/w illustrations, 28 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access