Mammary Stem Cells

Methods and Protocols

  • Maria del Mar Vivanco

Part of the Methods in Molecular Biology book series (MIMB, volume 1293)

Table of contents

  1. Front Matter
    Pages i-x
  2. Ming Luo, Shawn G. Clouthier, Yadwinder Deol, Suling Liu, Sunitha Nagrath, Ebrahim Azizi et al.
    Pages 1-49
  3. Oihana Iriondo, Miriam Rábano, María d. M. Vivanco
    Pages 63-72
  4. Fariba Behbod, Maria d. M. Vivanco
    Pages 73-81
  5. Ana M. Aransay, Laura Barcena, Aintzane Gonzalez-Lahera, Nuria Macias-Camara
    Pages 83-114
  6. René B. H. Braakman, Anieta M. Sieuwerts, Arzu Umar
    Pages 115-122
  7. Tommaso De Marchi, Eric Kuhn, Steven A. Carr, Arzu Umar
    Pages 123-135
  8. Richard Iggo, Elodie Richard
    Pages 137-160
  9. Marisa M. Faraldo, Marina A. Glukhova, Marie-Ange Deugnier
    Pages 161-172
  10. A. Wronski, L. M. Arendt, Charlotte Kuperwasser
    Pages 173-186
  11. Marc Guiu, Enrique J. Arenas, Sylwia Gawrzak, Milica Pavlovic, Roger R. Gomis
    Pages 221-229
  12. José Schneider
    Pages 267-271
  13. Back Matter
    Pages 273-275

About this book


The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.


breast cancer embryogenesis fluorescent activated cell sorting gene expression in vivo lineage tracing mammary gland metastasis regenerative medicine stem cells

Editors and affiliations

  • Maria del Mar Vivanco
    • 1
  1. 1.CIC bioGUNE, Technological Park of BizkaiaDerioSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2518-6
  • Online ISBN 978-1-4939-2519-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site