Mycobacteria Protocols

  • Tanya Parish
  • David M. Roberts

Part of the Methods in Molecular Biology book series (MIMB, volume 1285)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Andrej Benjak, Claudia Sala, Ruben C. Hartkoorn
    Pages 1-16
  3. Scarlet S. Shell, Michael R. Chase, Thomas R. Ioerger, Sarah M. Fortune
    Pages 31-45
  4. Megan C. Lucas, Lisa M. Wolfe, Rachel M. Hazenfield, Jade Kurihara, Nicole A. Kruh-Garcia, John Belisle et al.
    Pages 47-75
  5. Marie-Antoinette Lanéelle, Jérôme Nigou, Mamadou Daffé
    Pages 77-103
  6. Madhumitha Nandakumar, Gareth A. Prosser, Luiz Pedro S. de Carvalho, Kyu Rhee
    Pages 105-115
  7. Renan Goude, David M. Roberts, Tanya Parish
    Pages 117-130
  8. Krishnamoorthy Gopinath, Digby F. Warner, Valerie Mizrahi
    Pages 131-149
  9. Dirk Schnappinger, Kathryn M. O’Brien, Sabine Ehrt
    Pages 151-175
  10. Kenan C. Murphy, Kadamba Papavinasasundaram, Christopher M. Sassetti
    Pages 177-199
  11. Anil K. Ojha, William R. Jacobs Jr., Graham F. Hatfull
    Pages 215-226
  12. Liliana Rodrigues, Miguel Viveiros, José A. Aínsa
    Pages 227-239
  13. Delia Blanco-Ruano, David M. Roberts, Ruben Gonzalez-Del-Rio, Daniel Álvarez, Mª José Rebollo, Esther Pérez-Herrán et al.
    Pages 257-268

About this book


Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory.  Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites.  Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field, and to newcomers.


Genetic manipulation electroporation gene expression homologous recombination homologous recombineering lipid/glycolipid analysis macromolecules metabolites mycobacterial species whole genome sequencing

Editors and affiliations

  • Tanya Parish
    • 1
  • David M. Roberts
    • 2
  1. 1.Infectious Disease Research Inst (IDRI)SeattleUSA
  2. 2.Infectious Disease Research Inst (IDRI)SeattleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2449-3
  • Online ISBN 978-1-4939-2450-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book