Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1284)
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About this book
This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.
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Keywords
Table of contents (25 protocols)
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Front Matter
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Epigenetics
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Front Matter
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Transcriptional, Translational, and Metabolic Analysis
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Front Matter
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Forward and Reverse Genetics
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Plant Functional Genomics
Book Subtitle: Methods and Protocols
Editors: Jose M. Alonso, Anna N. Stepanova
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2444-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2443-1Published: 11 March 2015
Softcover ISBN: 978-1-4939-4913-7Published: 05 October 2016
eBook ISBN: 978-1-4939-2444-8Published: 11 March 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XV, 532
Number of Illustrations: 21 b/w illustrations, 64 illustrations in colour
Topics: Plant Sciences