PCR Primer Design

  • Chhandak Basu

Part of the Methods in Molecular Biology book series (MIMB, volume 1275)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Reidar Andreson, Lauris Kaplinski, Maido Remm
    Pages 1-16
  3. Alicia Rodríguez, Mar Rodríguez, Juan J. Córdoba, María J. Andrade
    Pages 31-56
  4. Kelvin Li, Susmita Shrivastava, Timothy B. Stockwell
    Pages 103-115
  5. Juliet O. Makanga, Antonius Christianto, Tetsuya Inazu
    Pages 117-125
  6. Lauris Kaplinski, Maido Remm
    Pages 127-142
  7. Anil Kumar, Nikita Chordia
    Pages 143-151
  8. Amarjeet Singh, Girdhar K. Pandey
    Pages 153-164
  9. Greiciane Gaburro Paneto, Francisco de Paula Careta
    Pages 165-172
  10. Brenda Thornton, Chhandak Basu
    Pages 173-179
  11. Garima Kushwaha, Gyan Prakash Srivastava, Dong Xu
    Pages 181-199
  12. Wubin Qu, Chenggang Zhang
    Pages 201-213
  13. Back Matter
    Pages 215-216

About this book


This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.


DNA amplification Polymerase Chain Reaction (PCR) degenerate PCR forensic biology genotyping in silico PCR primer designer multiplex PCR quantitative PCR

Editors and affiliations

  • Chhandak Basu
    • 1
  1. 1.Department of BiologyCalifornia State University, NorthridgeLos AngelesUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2364-9
  • Online ISBN 978-1-4939-2365-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site