RNA Remodeling Proteins

Methods and Protocols

  • Marc Boudvillain

Part of the Methods in Molecular Biology book series (MIMB, volume 1259)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Patrick Linder, Frances Fuller-Pace
    Pages 17-33
  3. Juan José García-Gómez, Simon Lebaron, Yves Henry, Jesús de la Cruz
    Pages 49-67
  4. Evan P. Booy, Ewan K. S. McRae, Sean A. McKenna
    Pages 125-135
  5. Wai Kit Ma, Elizabeth J. Tran
    Pages 183-197
  6. Atsushi Furuta, Kazi Abdus Salam, Hidenori Tani, Satoshi Tsuneda, Yuji Sekiguchi, Nobuyoshi Akimitsu et al.
    Pages 211-228
  7. Desiree Salas, Veronika Gocheva, Marcelo Nöllmann
    Pages 257-273
  8. Kimberly A. Reynolds, Veronica M. Raney, Kevin D. Raney
    Pages 275-291
  9. François D’Heygère, Annie Schwartz, Franck Coste, Bertrand Castaing, Marc Boudvillain
    Pages 293-311

About this book

Introduction

This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, RNA Remodeling Proteins: Methods and Protocols seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.

Keywords

DEAD-box Helicase EMOTE Fluorescence Resonance Energy Transfer (FRET) RHAU RNA Helicase RNA targets RNA unwinding molecular mechanisms proteins

Editors and affiliations

  • Marc Boudvillain
    • 1
  1. 1.CNRS Centre de Biophysique MoléculaireOrléansFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2214-7
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2213-0
  • Online ISBN 978-1-4939-2214-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book