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Glycosaminoglycans

Chemistry and Biology

  • Book
  • © 2015

Overview

Editors:
  1. Kuberan Balagurunathan

    1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, USA

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  2. Hiroshi Nakato

    1. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Umesh R. Desai

    1. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1229)

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Table of contents (48 protocols)

  1. Structure

    1. A Rapid, Nonradioactive Assay for Measuring Heparan Sulfate C-5 Epimerase Activity Using Hydrogen/Deuterium Exchange-Mass Spectrometry

      • Ponnusamy Babu, Xylophone V. Victor, Karthik Raman, Balagurunathan Kuberan
      Pages 209-219

    2. Aggrecan: Approaches to Study Biophysical and Biomechanical Properties

      • Hadi Tavakoli Nia, Christine Ortiz, Alan Grodzinsky
      Pages 221-237

    3. Use of Flow Cytometry for Characterization and Fractionation of Cell Populations Based on Their Expression of Heparan Sulfate Epitopes

      • Rebecca J. Holley, Raymond A. Smith, Els M. A. van de Westerlo, Claire E. Pickford, C. L. R. Merry, Toin H. van Kuppevelt
      Pages 239-251

    4. A Transgenic Approach to Live Imaging of Heparan Sulfate Modification Patterns

      • Matthew Attreed, Hannes E. Bülow
      Pages 253-268

  2. Functions

    1. Front Matter

      Pages 269-269
      Download chapter PDF

    2. Informatics Tools to Advance the Biology of Glycosaminoglycans and Proteoglycans

      • Lewis J. Frey
      Pages 271-287

    3. Designing “High-Affinity, High-Specificity” Glycosaminoglycan Sequences Through Computerized Modeling

      • Nehru Viji Sankaranarayanan, Aurijit Sarkar, Umesh R. Desai, Philip D. Mosier
      Pages 289-314

    4. Using Isothermal Titration Calorimetry to Determine Thermodynamic Parameters of Protein–Glycosaminoglycan Interactions

      • Amit K. Dutta, Jörg Rösgen, Krishna Rajarathnam
      Pages 315-324

    5. Characterizing Protein–Glycosaminoglycan Interactions Using Solution NMR Spectroscopy

      • Prem Raj B. Joseph, Krishna Mohan Poluri, Krishna Mohan Sepuru, Krishna Rajarathnam
      Pages 325-333

    6. Glycosaminoglycan–Protein Interaction Studies Using Fluorescence Spectroscopy

      • Rio S. Boothello, Rami A. Al-Horani, Umesh R. Desai
      Pages 335-353

    7. Studying Glycosaminoglycan–Protein Interactions Using Capillary Electrophoresis

      • Aiye Liang, Umesh R. Desai
      Pages 355-375

    8. Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain

      • Tomio Yabe, Nobuaki Maeda
      Pages 377-387

    9. Keratan Sulfate: Biosynthesis, Structures, and Biological Functions

      • Kenji Uchimura
      Pages 389-400

    10. The Sulfs: Expression, Purification, and Substrate Specificity

      • Kenji Uchimura
      Pages 401-412

    11. The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues

      • Marika Bogdani, Charmaine Simeonovic, Nadine Nagy, Pamela Y. Johnson, Christina K. Chan, Thomas N. Wight
      Pages 413-430

    12. Nonradioactive Glycosyltransferase and Sulfotransferase Assay to Study Glycosaminoglycan Biosynthesis

      • Cheryl M. Ethen, Miranda Machacek, Brittany Prather, Timothy Tatge, Haixiao Yu, Zhengliang L. Wu
      Pages 431-441

    13. Mapping Proteoglycan Functions with Glycosidases

      • Mauricio Cortes, Leslie K. Cortes, Nancy B. Schwartz
      Pages 443-455

    14. Cell Substrate Patterning with Glycosaminoglycans to Study Their Biological Roles in the Central Nervous System

      • Tony W. Hsiao, Vimal P. Swarup, Colin D. Eichinger, Vladimir Hlady
      Pages 457-467

    15. Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish

      • Fabienne E. Poulain
      Pages 469-482

    16. Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants

      • David Gailani, Qiufang Cheng, Ivan S. Ivanov
      Pages 483-496
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Keywords

About this book

Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches. Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Glycosaminoglycans: Chemistry and Biology serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans.

Editors and Affiliations

  • Department of Medicinal Chemistry, University of Utah, Salt Lake City, USA

    Kuberan Balagurunathan

  • Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, USA

    Hiroshi Nakato

  • Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, USA

    Umesh R. Desai

Bibliographic Information

  • Book Title: Glycosaminoglycans

  • Book Subtitle: Chemistry and Biology

  • Editors: Kuberan Balagurunathan, Hiroshi Nakato, Umesh R. Desai

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1714-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1713-6Published: 18 October 2014

  • Softcover ISBN: 978-1-4939-4696-9Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1714-3Published: 17 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 625

  • Number of Illustrations: 68 b/w illustrations, 72 illustrations in colour

  • Topics: Biochemistry, general

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