Protein Design

Methods and Applications

  • Valentin Köhler

Part of the Methods in Molecular Biology book series (MIMB, volume 1216)

Table of contents

  1. Front Matter
    Pages i-x
  2. Alexander Yakimov, Georgy Rychkov, Michael Petukhov
    Pages 1-14
  3. Phanourios Tamamis, Emmanouil Kasotakis, Georgios Archontis, Anna Mitraki
    Pages 53-70
  4. Benjamin C. Buer, E. Neil G. Marsh
    Pages 89-116
  5. Lucas B. Johnson, Thaddaus R. Huber, Christopher D. Snow
    Pages 129-159
  6. André C. Stiel, Kaspar Feldmeier, Birte Höcker
    Pages 183-196
  7. Matthew D. Smith, Alexandre Zanghellini, Daniela Grabs-Röthlisberger
    Pages 197-210
  8. Avanish S. Parmar, Douglas Pike, Vikas Nanda
    Pages 233-249
  9. Per Jr. Greisen, Sagar D. Khare
    Pages 265-273
  10. Back Matter
    Pages 275-279

About this book

Introduction

 Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.

Keywords

binary-patterned protein libraries fluorinated proteins functional sites of proteins ketol-acid reductoisomerase metals monomeric β-hairpin non-fluorinated analogous structures protein variants structure-forming elements symmetric protein folds synthetic unnatural amino acids β-sheet peptides

Editors and affiliations

  • Valentin Köhler
    • 1
  1. 1.Department of ChemistryUniversity of BaselSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1486-9
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1485-2
  • Online ISBN 978-1-4939-1486-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book