Regulatory Non-Coding RNAs

Methods and Protocols

  • Gordon G. Carmichael

Part of the Methods in Molecular Biology book series (MIMB, volume 1206)

Table of contents

  1. Front Matter
    Pages i-x
  2. Callie R. Merry, Courtney Niland, Ahmad M. Khalil
    Pages 1-14
  3. Dian-Jang Lee, Ernst Wagner, Taavi Lehto
    Pages 15-27
  4. Murugan Subramanian, Xiao Ling Li, Toshifumi Hara, Ashish Lal
    Pages 29-37
  5. Vidisha Tripathi, Jingyi Fei, Taekjip Ha, Kannanganattu V. Prasanth
    Pages 123-136
  6. John R. Sinnamon, Kevin Czaplinski
    Pages 137-148
  7. Back Matter
    Pages 179-181

About this book

Introduction

Regulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs, and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.

Keywords

Fluorescence in situ hybridization Long non-coding RNAs MicroRNA targets Non-coding transcriptome Protein-RNA interactions RNA-binding proteins siRNAs

Editors and affiliations

  • Gordon G. Carmichael
    • 1
  1. 1.University of Connecticut Health CenterFarmingtonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1369-5
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1368-8
  • Online ISBN 978-1-4939-1369-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book