Mass Spectrometry in Metabolomics

Methods and Protocols

  • Daniel Raftery

Part of the Methods in Molecular Biology book series (MIMB, volume 1198)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Historical and Methodology Overview

    1. Front Matter
      Pages 1-1
  3. Global LC-MS Based Metabolic Profiling

    1. Front Matter
      Pages 13-13
    2. Camilla B. Hill, Antony Bacic, Ute Roessner
      Pages 29-41
    3. Young-Mi Go, Karan Uppal, Douglas I. Walker, ViLinh Tran, Lauriane Dury, Frederick H. Strobel et al.
      Pages 43-73
    4. Antoni Beltran, Sara Samino, Oscar Yanes
      Pages 75-80
  4. Global GC-MS and CE-MS Based Metabolic Profiling

    1. Front Matter
      Pages 81-81
    2. Xue Shi, Xinmin Yin, Xiang Zhang
      Pages 99-105
    3. Antonia Garcia, Shama Naz, Coral Barbas
      Pages 107-123
  5. Isotope Labeling Methods

    1. Front Matter
      Pages 125-125
    2. Hans Fredrik Nyvold Kvitvang, Kåre A. Kristiansen, Stina K. Lien, Per Bruheim
      Pages 137-145
    3. Richard M. Higashi, Teresa W.-M. Fan, Pawel K. Lorkiewicz, Hunter N. B. Moseley, Andrew N. Lane
      Pages 147-167
  6. Targeted Aqueous Metabolite Profiling

    1. Front Matter
      Pages 169-169
    2. Ru Wei, Guodong Li, Albert B. Seymour
      Pages 171-199
  7. Lipid and Bile Acid Profiling Methods

    1. Front Matter
      Pages 201-201

About this book

Introduction

Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples, and this information provides enormous detail on biological systems and their current status.  Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance, and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.

Keywords

gas chromatography isotope labeling lipid profiling liquid chromatography mass spectrometry metabolic profiling

Editors and affiliations

  • Daniel Raftery
    • 1
  1. 1.University of Washington Fred Hutchinson Cancer Rsrch CtrSeattleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1258-2
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1257-5
  • Online ISBN 978-1-4939-1258-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book