Book Volume 1189 2015

Tissue Morphogenesis

Methods and Protocols

Editors:

ISBN: 978-1-4939-1163-9 (Print) 978-1-4939-1164-6 (Online)

Table of contents (22 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xii

  2. Imaging Morphogenesis

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-16

      Probing Regional Mechanical Properties of Embryonic Tissue Using Microindentation and Optical Coherence Tomography

    3. No Access

      Protocol

      Pages 17-30

      Hemodynamic Flow Visualization of Early Embryonic Great Vessels Using μPIV

    4. No Access

      Protocol

      Pages 31-46

      Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

    5. No Access

      Protocol

      Pages 47-61

      Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

    6. No Access

      Protocol

      Pages 63-78

      Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila melanogaster Embryos

    7. No Access

      Protocol

      Pages 79-97

      Light Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

    8. No Access

      Protocol

      Pages 99-113

      Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis

    9. No Access

      Protocol

      Pages 115-122

      A Multiplex Fluorescent In Situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

    10. No Access

      Protocol

      Pages 123-132

      Active Cell and ECM Movements During Development

  3. Culture Models of Morphogenesis

    1. Front Matter

      Pages 133-133

    2. No Access

      Protocol

      Pages 135-162

      3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

    3. No Access

      Protocol

      Pages 163-169

      Culture of Mouse Embryonic Foregut Explants

    4. No Access

      Protocol

      Pages 171-189

      Investigating Human Vascular Tube Morphogenesis and Maturation Using Endothelial Cell-Pericyte Co-cultures and a Doxycycline-Inducible Genetic System in 3D Extracellular Matrices

    5. No Access

      Protocol

      Pages 191-206

      Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues

  4. Manipulating Cells and Tissues In Vivo

    1. Front Matter

      Pages 207-207

    2. No Access

      Protocol

      Pages 209-218

      Probing Cell Mechanics with Subcellular Laser Dissection of Actomyosin Networks in the Early Developing Drosophila Embryo

    3. No Access

      Protocol

      Pages 219-235

      UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo In Vivo and Ex Vivo

    4. No Access

      Protocol

      Pages 237-254

      Measurement of Intercellular Cohesion by Tissue Surface Tensiometry

    5. No Access

      Protocol

      Pages 255-263

      Quail-Chick Chimeras and Eye Development

  5. Emerging Models of Tissue Morphogenesis

    1. Front Matter

      Pages 265-265

    2. No Access

      Protocol

      Pages 267-281

      Studying Epithelial Morphogenesis in Dictyostelium

    3. No Access

      Protocol

      Pages 283-297

      Primary Cell Cultures of Regenerating Holothurian Tissues

  6. Computational Models of Tissue Morphogenesis

    1. Front Matter

      Pages 299-299

    2. No Access

      Protocol

      Pages 301-322

      Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis Using CompuCell3D or VirtualLeaf

previous Page of 2