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Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Methods and Protocols

  • Bettina Warscheid

Part of the Methods in Molecular Biology book series (MIMB, volume 1188)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Silke Oeljeklaus, Andreas Schummer, Ida Suppanz, Bettina Warscheid
    Pages 23-46
  3. Guoan Zhang, Katrin Deinhardt, Thomas A. Neubert
    Pages 57-64
  4. Annemarie Matthes, Karin Köhl, Waltraud X. Schulze
    Pages 65-83
  5. Matthias D. Sury, Jia-Xuan Chen, Matthias Selbach
    Pages 85-93
  6. Soraya Hölper, Aaron Ruhs, Marcus Krüger
    Pages 95-106
  7. Julius Fredens, Kasper Engholm-Keller, Jakob Møller-Jensen, Martin Røssel Larsen, Nils J. Færgeman
    Pages 107-124
  8. Chiara Francavilla, Omid Hekmat, Blagoy Blagoev, Jesper V. Olsen
    Pages 125-148
  9. Zhiping Wu, Chan Hyun Na, Haiyan Tan, Junmin Peng
    Pages 149-160
  10. Ho-Tak Lau, Karen A. Lewis, Shao-En Ong
    Pages 161-175
  11. Karsten Boldt, Christian J. Gloeckner, Yves Texier, Felix von Zweydorf, Marius Ueffing
    Pages 177-190
  12. Carla Schmidt, Monika Raabe, Reinhard Lührmann, Henning Urlaub
    Pages 227-244
  13. Anders R. Kristensen, Leonard J. Foster
    Pages 263-270
  14. Andrea C. Becker, Jörn Dengjel
    Pages 271-279
  15. Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari, Tiziana Bonaldi
    Pages 293-311
  16. Jeanette Henningsen, Blagoy Blagoev, Irina Kratchmarova
    Pages 313-326
  17. Markus Kaller, Silke Oeljeklaus, Bettina Warscheid, Heiko Hermeking
    Pages 327-349
  18. Stefka Tyanova, Matthias Mann, Jürgen Cox
    Pages 351-364
  19. Back Matter
    Pages 365-372

About this book

Introduction

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.

Keywords

Cell Culture Gram negative bacteria Gram positive bacteria RNAi-mediated gene knockdown SILAC labeling of the yeast Saccharomyces cerevisiae SILAC technology Trypanosoma brucei autotrophic organisms human protein complexes human splicosome metabolic labeling nuclear protein-protein interactions protein interactomes quantitative phosphoproteome analyses in C. elegans signaling networks time-resolved phosphoproteomics studies

Editors and affiliations

  • Bettina Warscheid
    • 1
  1. 1.Institute of Biology IIUniversity of FreiburgFreiburgGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1142-4
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1141-7
  • Online ISBN 978-1-4939-1142-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site