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  • Book
  • © 2014

Hematopoietic Stem Cell Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1185)

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Table of contents (23 protocols)

  1. Transplantation Assays and Imaging Engraftment

    1. Front Matter

      Pages 235-235
    2. Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells

      • Ryo Yamamoto, Yohei Morita, Hiromitsu Nakauchi
      Pages 237-245
    3. Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull

      • Juwell W. Wu, Judith M. Runnels, Charles P. Lin
      Pages 247-265
    4. Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development

      • Ken-Edwin Aryee, Leonard D. Shultz, Michael A. Brehm
      Pages 267-278
    5. Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells

      • Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, Linheng Li
      Pages 279-284
  2. Genetic Modification

    1. Front Matter

      Pages 285-285
    2. Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells

      • Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, David A. Williams
      Pages 287-309
    3. Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells

      • Niek P. van Til, Gerard Wagemaker
      Pages 311-319
    4. High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies

      • Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, Hans-Peter Kiem
      Pages 321-344
    5. Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells

      • Leonid V. Bystrykh, Gerald de Haan, Evgenia Verovskaya
      Pages 345-360
  3. Back Matter

    Pages 361-364

About this book

Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation.

Editors and Affiliations

  • Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, USA

    Kevin D. Bunting

  • Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, USA

    Cheng-Kui Qu

Bibliographic Information

  • Book Title: Hematopoietic Stem Cell Protocols

  • Editors: Kevin D. Bunting, Cheng-Kui Qu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1133-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1132-5Published: 26 July 2014

  • Softcover ISBN: 978-1-4939-4757-7Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1133-2Published: 25 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 364

  • Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour

  • Topics: Cell Biology, Stem Cells

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access