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Patch-Clamp Methods and Protocols

  • Marzia Martina
  • Stefano Taverna

Part of the Methods in Molecular Biology book series (MIMB, volume 1183)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Alexei Verkhratsky, Vladimir Parpura
    Pages 1-19
  3. Pharmacology

  4. Physiology

    1. Front Matter
      Pages 115-115
    2. Enrica Maria Petrini, Andrea Barberis
      Pages 117-141
    3. Romain Lefebvre, Sandrine Pouvreau, Claude Collet, Bruno Allard, Vincent Jacquemond
      Pages 159-170
    4. Joshua L. Plotkin, D. James Surmeier
      Pages 171-182
    5. Cary Soares, Kevin F. H. Lee, Denise Cook, Jean-Claude Béïque
      Pages 205-219
    6. Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, James J. Hickman
      Pages 243-252
    7. Peter Molnar, James J. Hickman
      Pages 253-261
    8. Albert K. Lee, Jérôme Epsztein, Michael Brecht
      Pages 263-276
  5. Biophysics

    1. Front Matter
      Pages 277-277
    2. Romain Guinamard, Thomas Hof, Laurent Sallé
      Pages 291-307
    3. Géza Berecki, Arie O. Verkerk, Antoni C. G. van Ginneken, Ronald Wilders
      Pages 309-326
    4. Francis A. Ortega, Robert J. Butera, David J. Christini, John A. White, Alan D. Dorval II
      Pages 327-354
  6. Back Matter
    Pages 401-403

About this book

Introduction

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies, and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology, and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals, and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Patch

-Clamp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

Keywords

biophysics dynamic clamp patch-clamp pharmacology physiology

Editors and affiliations

  • Marzia Martina
    • 1
  • Stefano Taverna
    • 2
  1. 1.Department of Translational BioscienceNational Research Council of CanadaOttawaCanada
  2. 2.Dept of Neuroscience & Brain TechnologiesItalian Institute of TechnologyGenoaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1096-0
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1095-3
  • Online ISBN 978-1-4939-1096-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site