Protein Affinity Tags

Methods and Protocols

  • Richard J. Giannone
  • Andrew B. Dykstra

Part of the Methods in Molecular Biology book series (MIMB, volume 1177)

Table of contents

  1. Front Matter
    Pages i-x
  2. Wesley B. Asher, Kara L. Bren
    Pages 17-33
  3. Dennis Kuo, Minghua Nie, Albert J. Courey
    Pages 71-80
  4. Yoshimitsu Takakura, Naomi Oka, Masako Tsunashima
    Pages 95-106
  5. Silvia C. Locatelli-Hoops, Alexei A. Yeliseev
    Pages 107-120
  6. Takashi Murayama, Takuya Kobayashi
    Pages 151-161
  7. Ursula Schäffer, Ralf Baumeister, Ekkehard Schulze
    Pages 163-174
  8. Hanhui Ma, Janel R. McLean, Kathleen L. Gould, Dannel McCollum
    Pages 175-191
  9. Back Matter
    Pages 211-213

About this book


Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.


affinity purification fluorescent proteins protein protein interactions protein purification recombinant proteins

Editors and affiliations

  • Richard J. Giannone
    • 1
  • Andrew B. Dykstra
    • 2
  1. 1.Organic & Biological Mass Spectrometry, Chemical Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  2. 2.Analytical SciencesAmgenWest GreenwichUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1033-5
  • Online ISBN 978-1-4939-1034-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site