Axon Growth and Regeneration

Methods and Protocols

  • Andrew J.¬†Murray

Part of the Methods in Molecular Biology book series (MIMB, volume 1162)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Review

    1. Front Matter
      Pages 1-1
    2. Andrew J. Murray
      Pages 3-14
  3. Monitoring Intracellular Pathways in Growth Cones

  4. Assessing Axonal Responses to Extracellular Factors In Vitro

    1. Front Matter
      Pages 41-41
    2. Andrew Kaplan, Ricardo Sanz, Gino B. Ferraro, Ricardo Alchini, Alyson E. Fournier
      Pages 43-56
    3. Tom Buyens, Djoere Gaublomme, Inge Van Hove, Lies De Groef, Lieve Moons
      Pages 57-71
    4. Geoffrey M. W. Cook, Prem Jareonsettasin, Roger J. Keynes
      Pages 73-83
    5. Jaewon Park, Sunja Kim, Jianrong Li, Arum Han
      Pages 85-95
  5. Axon Injury and Regeneration In Vivo

    1. Front Matter
      Pages 125-125
    2. Jessica C. F. Kwok, Janosch P. Heller, Rong-Rong Zhao, James W. Fawcett
      Pages 127-138
    3. Suelen Adriani Marques, Fernanda Martins de Almeida, Klauss Mostacada, Ana Maria Blanco Martinez
      Pages 149-156
    4. Jacob Koffler, Ramsey F. Samara, Ephron S. Rosenzweig
      Pages 157-165
  6. Assessing the Extent of Axon Regeneration

    1. Front Matter
      Pages 177-177

About this book

Introduction

Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies.  After an introductory section, this detailed volume continues with sections focusing on axon growth in vitro, providing a range of protocols that can be used to examine intracellular signalling pathways, axonal responses to extracellular factors, and methods for quantifying outgrowth. The next section provides protocols for inducing experimental injury in vivo as well as some highly promising protocols for promoting regeneration, which segues into the final section highlighting a series of protocols that can be used to monitor the extent of axon regeneration in vivo, ranging from tract tracing to in vivo imaging and functional recovery. As a book in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and reliable, Axon Growth and Regeneration: Methods and Protocols aims to serve researchers studying axon regeneration with a significant set of diverse tools, vital for moving on to the next generation of exciting new discoveries in the field.

Keywords

Axon growth Axon repair Axonal responses Central nervous system repair Experimental injury induction Intracellular signalling pathways

Editors and affiliations

  • Andrew J.¬†Murray
    • 1
  1. 1.Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0777-9
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0776-2
  • Online ISBN 978-1-4939-0777-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book